Details

Autor(en) / Beteiligte

• Sowers, Molly A
• McCombs, Jessica R
• Wang, Ying
• Paletta, Joseph T
• Morton, Stephen W
• Dreaden, Erik C
• Boska, Michael D
• Ottaviani, M Francesca
• Hammond, Paula T
• Rajca, Andrzej
• Johnson, Jeremiah A

Titel

Redox-responsive branched-bottlebrush polymers for in vivo MRI and fluorescence imaging

Ist Teil von

• Nature communications, 2014-11, Vol.5, p.5460-5460

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Stimuli-responsive multimodality imaging agents have broad potential in medical diagnostics. Herein, we report the development of a new class of branched-bottlebrush polymer dual-modality organic radical contrast agents--ORCAFluors--for combined magnetic resonance and near-infrared fluorescence imaging in vivo. These nitroxide radical-based nanostructures have longitudinal and transverse relaxation times that are on par with commonly used heavy-metal-based magnetic resonance imaging (MRI) contrast agents. Furthermore, these materials display a unique compensatory redox response: fluorescence is partially quenched by surrounding nitroxides in the native state; exposure to ascorbate or ascorbate/glutathione leads to nitroxide reduction and a concomitant 2- to 3.5-fold increase in fluorescence emission. This behaviour enables correlation of MRI contrast, fluorescence intensity and spin concentration with tissues known to possess high concentrations of ascorbate in mice. Our in vitro and in vivo results, along with our modular synthetic approach, make ORCAFluors a promising new platform for multimodality molecular imaging.