Details

Autor(en) / Beteiligte

• Matranga, Christian B
• Andersen, Kristian G
• Winnicki, Sarah
• Busby, Michele
• Gladden, Adrianne D
• Tewhey, Ryan
• Stremlau, Matthew
• Berlin, Aaron
• Gire, Stephen K
• England, Eleina
• Moses, Lina M
• Mikkelsen, Tarjei S
• Odia, Ikponmwonsa
• Ehiane, Philomena E
• Folarin, Onikepe
• Goba, Augustine
• Kahn, S Humarr
• Grant, Donald S
• Honko, Anna
• Hensley, Lisa
• Happi, Christian
• Garry, Robert F
• Malboeuf, Christine M
• Birren, Bruce W
• Gnirke, Andreas
• Levin, Joshua Z
• Sabeti, Pardis C

Titel

Enhanced methods for unbiased deep sequencing of Lassa and Ebola RNA viruses from clinical and biological samples

Ist Teil von

• Genome biology, 2014-01, Vol.15 (11), p.519-519, Article 519

Ort / Verlag

England: BioMed Central Ltd

Erscheinungsjahr

2014

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• We have developed a robust RNA sequencing method for generating complete de novo assemblies with intra-host variant calls of Lassa and Ebola virus genomes in clinical and biological samples. Our method uses targeted RNase H-based digestion to remove contaminating poly(rA) carrier and ribosomal RNA. This depletion step improves both the quality of data and quantity of informative reads in unbiased total RNA sequencing libraries. We have also developed a hybrid-selection protocol to further enrich the viral content of sequencing libraries. These protocols have enabled rapid deep sequencing of both Lassa and Ebola virus and are broadly applicable to other viral genomics studies.