Details

Autor(en) / Beteiligte

• Melo, Sonia A.
• Sugimoto, Hikaru
• O’Connell, Joyce T.
• Kato, Noritoshi
• Villanueva, Alberto
• Vidal, August
• Qiu, Le
• Vitkin, Edward
• Perelman, Lev T.
• Melo, Carlos A.
• Lucci, Anthony
• Ivan, Cristina
• Calin, George A.
• Kalluri, Raghu

Titel

Cancer Exosomes Perform Cell-Independent MicroRNA Biogenesis and Promote Tumorigenesis

Ist Teil von

• Cancer cell, 2014-11, Vol.26 (5), p.707-721

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Exosomes are secreted by all cell types and contain proteins and nucleic acids. Here, we report that breast cancer associated exosomes contain microRNAs (miRNAs) associated with the RISC-Loading Complex (RLC) and display cell-independent capacity to process precursor microRNAs (pre-miRNAs) into mature miRNAs. Pre-miRNAs, along with Dicer, AGO2, and TRBP, are present in exosomes of cancer cells. CD43 mediates the accumulation of Dicer specifically in cancer exosomes. Cancer exosomes mediate an efficient and rapid silencing of mRNAs to reprogram the target cell transcriptome. Exosomes derived from cells and sera of patients with breast cancer instigate nontumorigenic epithelial cells to form tumors in a Dicer-dependent manner. These findings offer opportunities for the development of exosomes based biomarkers and therapies. •Cancer exosomes use Dicer, TRBP, and AGO2 to process pre-miRNAs to generate miRNAs•CD43 mediates accumulation of Dicer specifically in cancer exosomes•Cancer exosomes alter the transcriptome of target cells in a Dicer-dependent manner•Breast cancer serum exosomes process pre-miRNAs into miRNAs inducing tumor formation Melo et al. report that breast cancer cells secrete exosomes with CD43-mediated accumulation of Dicer and capacity for cell-independent microRNA biogenesis. Cancer exosomes promote tumor formation of nontumorigenic epithelial cells by altering the transcriptome of target cells.