Details

Autor(en) / Beteiligte

• Issa, John B.
• Haeffele, Benjamin D.
• Agarwal, Amit
• Bergles, Dwight E.
• Young, Eric D.
• Yue, David T.

Titel

Multiscale Optical Ca2+ Imaging of Tonal Organization in Mouse Auditory Cortex

Ist Teil von

• Neuron (Cambridge, Mass.), 2014-08, Vol.83 (4), p.944-959

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Spatial patterns of functional organization, resolved by microelectrode mapping, comprise a core principle of sensory cortices. In auditory cortex, however, recent two-photon Ca2+ imaging challenges this precept, as the traditional tonotopic arrangement appears weakly organized at the level of individual neurons. To resolve this fundamental ambiguity about the organization of auditory cortex, we developed multiscale optical Ca2+ imaging of unanesthetized GCaMP transgenic mice. Single-neuron activity monitored by two-photon imaging was precisely registered to large-scale cortical maps provided by transcranial widefield imaging. Neurons in the primary field responded well to tones; neighboring neurons were appreciably cotuned, and preferred frequencies adhered tightly to a tonotopic axis. By contrast, nearby secondary-field neurons exhibited heterogeneous tuning. The multiscale imaging approach also readily localized vocalization regions and neurons. Altogether, these findings cohere electrode and two-photon perspectives, resolve new features of auditory cortex, and offer a promising approach generalizable to any cortical area. •High-sensitivity mode of transcranial imaging of cortex in unanesthetized mice•Spectral organization of auditory cortex under widefield imaging is highly regular•Neighboring neurons in AI are appreciably cotuned•Increased spectral integration is observed in neurons of AII Understanding cortical organization across large-to-small spatial scales is critical to brain mapping. Issa et al. develop an imaging approach in GCaMP-expressing mice to characterize sound responses of auditory cortex from the level of individual neurons up to large-scale cortical maps.