Details

Autor(en) / Beteiligte

• Koido, Shigeo
• Ohkusa, Toshifumi
• Kan, Shin
• Takakura, Kazuki
• Saito, Keisuke
• Komita, Hideo
• Ito, Zensho
• Kobayashi, Hiroko
• Takami, Shinichiro
• Uchiyama, Kan
• Arakawa, Hiroshi
• Ito, Masaki
• Okamoto, Masato
• Kajihara, Mikio
• Homma, Sadamu
• Tajiri, Hisao

Titel

Production of corticotropin-releasing factor and urocortin from human monocyte-derived dendritic cells is stimulated by commensal bacteria in intestine

Ist Teil von

• World journal of gastroenterology : WJG, 2014-10, Vol.20 (39), p.14420-14429

Ort / Verlag

United States: Baishideng Publishing Group Inc

Erscheinungsjahr

2014

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• AIM:To examine whether commensal bacteria are a contributing cause of stress-related mucosal inflammation.METHODS:Human peripheral blood monocyte-deriveddendritic cells(MoDCs)were stimulated by commensal bacterial strains,including Escherichia coli,Clostridium clostridioforme,Bacteroides vulgatus(B.vulgatus),Fusobacterium varium(F.varium),and Lactobacillus delbrueckii subsp.bulgaricus.After incubation,corticotropin-releasing factor(CRF)and urocortin 1(UCN1)mRNA in the cells was examined by real-time reverse transcription polymerase chain reaction.Supernatants from the cells were tested for CRF and UCN1 using an enzyme-linked immunosorbent assay.RESULTS:Both CRF and UCN1 were significantly augmented by B.vulgatus and F.varium at both the mRNA and protein levels.In particular,B.vulgatus stimulated human MoDCs,resulting in extremely high levels of CRF and UCN1.CONCLUSION:Stimulation of MoDCs by B.vulgatus and F.varium may be associated with CRF/UCN1-related intestinal disorders,such as irritable bowel syndrome and inflammatory bowel disease.