Details

Autor(en) / Beteiligte

• Harrison, Melissa M
• Jenkins, Brian V
• O'Connor-Giles, Kate M
• Wildonger, Jill

Titel

A CRISPR view of development

Ist Teil von

• Genes & development, 2014-09, Vol.28 (17), p.1859-1872

Ort / Verlag

United States: Cold Spring Harbor Laboratory Press

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek

Beschreibungen/Notizen

• The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated nuclease 9) system is poised to transform developmental biology by providing a simple, efficient method to precisely manipulate the genome of virtually any developing organism. This RNA-guided nuclease (RGN)-based approach already has been effectively used to induce targeted mutations in multiple genes simultaneously, create conditional alleles, and generate endogenously tagged proteins. Illustrating the adaptability of RGNs, the genomes of >20 different plant and animal species as well as multiple cell lines and primary cells have been successfully modified. Here we review the current and potential uses of RGNs to investigate genome function during development.