Acta pharmacologica Sinica, 2014-10, Vol.35 (10), p.1311-1322
2014
Details
- Autor(en) / Beteiligte
- Titel
- Telekin suppresses human hepatocellular carcinoma cells in vitro by inducing G2/M phase arrest via the p38 MAPK signaling pathway
- Ist Teil von
- Acta pharmacologica Sinica, 2014-10, Vol.35 (10), p.1311-1322
- Ort / Verlag
- United States: Nature Publishing Group
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- Aim： Telekin, isolated from the Chinese herb Carpesium divaricatum, has shown anti-proliferation effects against various cancer cells, including hepatocellular carcinoma cells. In this study, we investigated the anti-proliferation mechanisms of telekin in human hepatocellular carcinoma HepG2 cells in vitro. Methods： HepG2 cells were treated with telekin. Cell viability was evaluated using M＇FF assay. Flow cytometry was used to measure cell cycle profiles, ROS level and apoptosis. The protein expression levels were analyzed with Western blotting. Results： Telekin （3.75-30 pmol/L） dose-dependently inhibited the viability of HepG2 cells and induced I apoptosis. Furthermore, the treatment induced cell cycle arrest at G2/M phase, accompanied by significantly increased the phosphorylation of Cdc25A and Cdc2, and decreased Cyclin B1 level. Moreover, the treatment significantly stimulated ROS production, and increased the phosphorylation of p38 and MAPKAPK-2 in the cells. Pretreatment with the antioxidant NAC （2.5, 5, and 10 mmol/L）, or the p38 MAPK inhibitor SB203580 （2.5 and 5 pmol/L） dose-dependently attenuated these telekin-induced effects in the cells. Conclusion： Telekin suppresses hepatocellular carcinoma cells in vitro by inducing G2/M phase arrest via activating the p38 MAPK pathway.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1671-4083eISSN: 1745-7254DOI: 10.1038/aps.2014.74Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4186990
- Format
- –
- Schlagworte
- Apoptosis - drug effects, Carcinoma, Hepatocellular - drug therapy, Carcinoma, Hepatocellular - metabolism, CDC2 Protein Kinase, cdc25 Phosphatases - metabolism, Cell Cycle Checkpoints - drug effects, Cell Line, Tumor, Cell Proliferation - drug effects, Cell Survival - drug effects, Cyclin B1 - metabolism, Cyclin-Dependent Kinases - metabolism, G2 Phase Cell Cycle Checkpoints - drug effects, Hep G2 Cells, HepG2细胞, Humans, Intracellular Signaling Peptides and Proteins - metabolism, Liver Neoplasms - drug therapy, Liver Neoplasms - metabolism, MAPK信号通路, Original, P38, p38 Mitogen-Activated Protein Kinases - metabolism, Protein-Serine-Threonine Kinases - metabolism, Reactive Oxygen Species - metabolism, Sesquiterpenes, Eudesmane - pharmacology, Signal Transduction - drug effects, Western印迹, 体外, 肝癌细胞, 诱导