Details

Autor(en) / Beteiligte

• Huetz, F.
• Carlsson, L.
• Tornberg, U.C.
• Holmberg, D.

Titel

V‐region directed selection in differentiating B lymphocytes

Ist Teil von

• The EMBO journal, 1993-05, Vol.12 (5), p.1819-1826

Ort / Verlag

London: Nature Publishing Group

Erscheinungsjahr

1993

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• We here analyse the repertoire of VH7183 rearrangements isolated from different stages of B cell differentiation in adult mice. The nucleotide sequence analyses of VH7183‐D‐JH rearrangements derived from large pre‐B cells (B220+, mu‐), small pre‐B cells (B220+, mu‐) and mature B cells (B220+, mu+) isolated from adult bone marrow revealed a sequential accumulation, among functional rearrangements, of D segments of the FL16 family and a depletion of D segments using the second and the third reading frame (RF). One member (VH7183.1) of the VH7183 gene family was utilized in 60–80% of the rearrangements of all populations analysed. In neonates the majority of the rearrangements utilizing this gene was found to be functional. In contrast, > 96% of the VH7183 rearrangements isolated from adult spleen were non‐functional. These data provide evidence for cellular selection of VH regions acting at different points of the B cell differentiation pathway and at the transition of B cells from the bone marrow to the periphery.