Details

Autor(en) / Beteiligte

• Jiang, Yong-Jian
• Lee, Chong-Lek
• Wang, Qiang
• Zhou, Zhong-Wen
• Yang, Feng
• Jin, Chen
• Fu, De-Liang

Titel

Establishment of an orthotopic pancreatic cancer mouse model: Cells suspended and injected in Matrigel

Ist Teil von

• World journal of gastroenterology : WJG, 2014-07, Vol.20 (28), p.9476-9485

Ort / Verlag

United States: Baishideng Publishing Group Inc

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• AIM:To establish an orthotopic mouse model of pancreatic cancer that mimics the pathological features of exocrine pancreatic adenocarcinoma.METHODS:Pan02 cells were suspended in low-temperature Matrigel and injected into the parenchyma of pancreatic tails of C57BL/6 mice,with cells suspended in phosphate buffered saline(PBS)serving as a control.Primary and implanted tumors were confirmed pathologically.The rate of tumor formation and intraperitoneal implantation in the two groups were compared at different time points after injection.Leakage and intraabdominal dispersion of Matrigel and PBS,both dyed with methylene blue,were compared after injection into the parenchyma of the pancreas.We observed adherence and proliferation in Pan02 cells suspended in Matrigel in vitro.We also compared the pathological manifestation of this orthotopic pancreatic cancer model in the head and tails of the pancreas.The characteristics of the origin of epithelial cells and exocrine markers of established orthotopic pancreatic tumors were confirmed using immunohistochemistry.RESULTS:Diluted Matrigel could form a gel drip in the pancreatic parenchyma,effectively preventing leakage from the injection site and avoiding dispersion in the abdominal cavity.Pan02 cells were able to adhere to a dish,proliferate,and migrate in the gel drip.The tumor formation rate in the Matrigel group was 100%at both2 and 3 wk after injection,whereas it was 25.0%and37.5%in the PBS group at 2 and 3 wk,respectively(P<0.05).The intraperitoneal tumor implantation rate was 75.0%in the PBS group after 3 wk of injection,while it was 12.5%in the Matrigel group(P<0.05).Hepatoduodenal ligament and duodenal invasions with obstructive jaundice and upper digestive obstruction with mesenteric lymph node metastasis were observed in the pancreatic head group.In the pancreatic tail group,spleen and gastric invasion were dominant,leading to retroperitoneal lymph nodes metastasis.Positive immunohistochemical staining of cytokeratin and negative staining of vimentin and chromogranin A confirmed that the orthotopic pancreatic tumor injected with Pan02 cells suspended in Matrigel was of epithelial origin and expressed exocrine markers of cancer.CONCLUSION:This method of low-temperature Matrigel suspension and injection is effective for establishing an orthotopic mouse model of pancreatic cancer.