Details

Autor(en) / Beteiligte

• Abolhassani, Ali
• Riazi, Gholam Hossein
• Azizi, Ebrahim
• Amanpour, Saeid
• Muhammadnejad, Samad
• Haddadi, Mahnaz
• Zekri, Ali
• Shirkoohi, Reza

Titel

FGF10: Type III Epithelial Mesenchymal Transition and Invasion in Breast Cancer Cell Lines

Ist Teil von

• Journal of Cancer, 2014-01, Vol.5 (7), p.537-547

Ort / Verlag

Australia: Ivyspring International Publisher

Erscheinungsjahr

2014

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Fibroblastic growth factor-10 (FGF-10) has an important role in type I epithelial mesenchymal transition (EMT) during the embryonic period of life (gastrulation). Since EMT has a critical role during cancer cells invasion and metastasis (type III) this study sought to investigate the possible role of FGF-10 in type III EMT by monitoring breast cancer cell lines' behavior by FGF-10 regulation. MCF-7 and MDA-MB-231 cell lines with different levels of FGF10 expression were treated with FGF-10 recombinant protein and FGF-10 siRNA, respectively. The cell viability, migration, colony formation and wound healing have a direct relationship with FGF-10 expression, while FGF-10 expression decreased apoptosis. All mesenchymal factors (such as vimentin, N cadherin, snail, slug, TGF-β) increased due to FGF-10 expression with contrary expression of epithelial markers (such as E-cadherin). Moreover, GSK3β phosphorylation (inactivation) increased with FGF-10 expression. The important role of FGF-10 in type III EMT on cancer cells and initiation of metastasis via various kinds of signaling pathways has been suggested.