The FASEB journal, 2012-02, Vol.26 (2), p.567-575
2012
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- Autor(en) / Beteiligte
- Titel
- Heat treatment of retinal pigment epithelium induces production of elastic lamina components and antiangiogenic activity
- Ist Teil von
- The FASEB journal, 2012-02, Vol.26 (2), p.567-575
- Ort / Verlag
- United States: Federation of American Societies for Experimental Biology
- Erscheinungsjahr
- 2012
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- ABSTRACT Age‐related macular degeneration (AMD) is the leading cause of blindness in the Western world. In advanced AMD, new vessels from choriocapillaris (CC) invade through the Bruch's membrane (BrM) into the retina, forming choroidal neovascularization (CNV). BrM, an elastic lamina that is located between the retinal pigment epithelium (RPE) and CC, is thought to act as a physical and functional barrier against CNV. The BrM of patients with early AMD are characterized by decreased levels of antiangiogenic factors, including endostatin, thrombospondin‐1 (TSP‐1), and pigment epithelium‐derived factor (PEDF), as well as by degeneration of the elastic layer. Motivated by a previous report that heat increases elastin expression in human skin, we examined the effect of heat on human ARPE‐19 cell production of BrM components. Heat treatment stimulated the production of BrM components, including TSP‐1, PEDF, and tropoelastin in vitro and increased the antiangiogenic activity of RPE measured in a mouse corneal pocket assay. The effect of heat on experimental CNV was investigated by pretreating the retina with heat via infrared diode laser prior to the induction of CNV. Heat treatment blocked the development of experimental CNV in vivo. These findings suggest that heat treatment may restore BrM integrity and barrier function against new vessel growth.—Sekiyama, E., Saint‐Geniez, M., Yoneda, K., Hisatomi, T., Nakao, S., Walshe, T. E., Maruyama, K., Hafezi‐Moghadam, A., Miller, J. W., Kinoshita, S., D'Amore, P. A. Heat treatment of retinal pigment epithelium induces production of elastic lamina components and anti‐angiogenic activity. FASEB J. 26, 567–575 (2012). www.fasebj.org
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0892-6638eISSN: 1530-6860DOI: 10.1096/fj.11-184127Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4090388
- Format
- –
- Schlagworte
- Angiogenesis Inhibitors - genetics, Angiogenesis Inhibitors - metabolism, Animals, Bruch Membrane - blood supply, Bruch Membrane - metabolism, Bruch Membrane - pathology, Cell Line, choroidal neovascularization, Choroidal Neovascularization - genetics, Choroidal Neovascularization - metabolism, Choroidal Neovascularization - pathology, Choroidal Neovascularization - prevention & control, Elastic Tissue - metabolism, Elastic Tissue - pathology, elastin, endostatin, Endostatins - genetics, Endostatins - metabolism, Eye Proteins - genetics, Eye Proteins - metabolism, Gene Expression, Hot Temperature - therapeutic use, Humans, Lasers, Semiconductor - therapeutic use, Macular Degeneration - genetics, Macular Degeneration - metabolism, Macular Degeneration - pathology, Macular Degeneration - therapy, Mice, Mice, Inbred C57BL, Nerve Growth Factors - genetics, Nerve Growth Factors - metabolism, Research Communications, Retinal Pigment Epithelium - blood supply, Retinal Pigment Epithelium - metabolism, Retinal Pigment Epithelium - pathology, RNA, Messenger - genetics, RNA, Messenger - metabolism, Serpins - genetics, Serpins - metabolism, Thrombospondin 1 - genetics, Thrombospondin 1 - metabolism, thrombospondin‐1, transpupillary thermotherapy, Tropoelastin - metabolism, Wet Macular Degeneration - genetics, Wet Macular Degeneration - metabolism, Wet Macular Degeneration - pathology, Wet Macular Degeneration - prevention & control