Details

Autor(en) / Beteiligte

• Mascher, Martin
• Jost, Matthias
• Kuon, Joel-Elias
• Himmelbach, Axel
• Aßfalg, Axel
• Beier, Sebastian
• Scholz, Uwe
• Graner, Andreas
• Stein, Nils

Titel

Mapping-by-sequencing accelerates forward genetics in barley

Ist Teil von

• Genome biology, 2014-06, Vol.15 (6), p.R78-R78

Ort / Verlag

England: BioMed Central

Erscheinungsjahr

2014

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Mapping-by-sequencing has emerged as a powerful technique for genetic mapping in several plant and animal species. As this resequencing-based method requires a reference genome, its application to complex plant genomes with incomplete and fragmented sequence resources remains challenging. We perform exome sequencing of phenotypic bulks of a mapping population of barley segregating for a mutant phenotype that increases the rate of leaf initiation. Read depth analysis identifies a candidate gene, which is confirmed by the analysis of independent mutant alleles. Our method illustrates how the genomic resources of barley together with exome resequencing can underpin mapping-by-sequencing.