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Mapping-by-sequencing accelerates forward genetics in barley
Ist Teil von
Genome biology, 2014-06, Vol.15 (6), p.R78-R78
Ort / Verlag
England: BioMed Central
Erscheinungsjahr
2014
Quelle
EZB-FREE-00999 freely available EZB journals
Beschreibungen/Notizen
Mapping-by-sequencing has emerged as a powerful technique for genetic mapping in several plant and animal species. As this resequencing-based method requires a reference genome, its application to complex plant genomes with incomplete and fragmented sequence resources remains challenging. We perform exome sequencing of phenotypic bulks of a mapping population of barley segregating for a mutant phenotype that increases the rate of leaf initiation. Read depth analysis identifies a candidate gene, which is confirmed by the analysis of independent mutant alleles. Our method illustrates how the genomic resources of barley together with exome resequencing can underpin mapping-by-sequencing.