Development (Cambridge), 2012-05, Vol.139 (10), p.1831-1841
2012
Details
- Autor(en) / Beteiligte
- Titel
- Bcl11a is required for neuronal morphogenesis and sensory circuit formation in dorsal spinal cord development
- Ist Teil von
- Development (Cambridge), 2012-05, Vol.139 (10), p.1831-1841
- Ort / Verlag
- England
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Dorsal spinal cord neurons receive and integrate somatosensory information provided by neurons located in dorsal root ganglia. Here we demonstrate that dorsal spinal neurons require the Krüppel-C(2)H(2) zinc-finger transcription factor Bcl11a for terminal differentiation and morphogenesis. The disrupted differentiation of dorsal spinal neurons observed in Bcl11a mutant mice interferes with their correct innervation by cutaneous sensory neurons. To understand the mechanism underlying the innervation deficit, we characterized changes in gene expression in the dorsal horn of Bcl11a mutants and identified dysregulated expression of the gene encoding secreted frizzled-related protein 3 (sFRP3, or Frzb). Frzb mutant mice show a deficit in the innervation of the spinal cord, suggesting that the dysregulated expression of Frzb can account in part for the phenotype of Bcl11a mutants. Thus, our genetic analysis of Bcl11a reveals essential functions of this transcription factor in neuronal morphogenesis and sensory wiring of the dorsal spinal cord and identifies Frzb, a component of the Wnt pathway, as a downstream acting molecule involved in this process.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0950-1991eISSN: 1477-9129DOI: 10.1242/dev.072850Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4067532
- Format
- –
- Schlagworte
- Animals, Carrier Proteins - genetics, Carrier Proteins - metabolism, Cell Differentiation - genetics, Cell Differentiation - physiology, Cells, Cultured, Chromatin Immunoprecipitation, Circuits, Differentiation, Dorsal horn, Dorsal root ganglia, Electrophysiology, Frizzled-related protein 3, Ganglia, Spinal - cytology, Ganglia, Spinal - metabolism, Gene expression, Genetic analysis, In Situ Hybridization, Information processing, Innervation, Mice, Mice, Knockout, Morphogenesis, Morphogenesis - genetics, Morphogenesis - physiology, Neurons - cytology, Neurons - metabolism, Nuclear Proteins - genetics, Nuclear Proteins - metabolism, Real-Time Polymerase Chain Reaction, Sensory neurons, Sensory Receptor Cells - cytology, Sensory Receptor Cells - metabolism, Spinal cord, Spinal Cord - cytology, Spinal Cord - metabolism, Transcription factors, Wnt protein