Details

Autor(en) / Beteiligte

• Xiang, Jian-Feng
• Yin, Qing-Fei
• Chen, Tian
• Zhang, Yang
• Zhang, Xiao-Ou
• Wu, Zheng
• Zhang, Shaofeng
• Wang, Hai-Bin
• Ge, Junhui
• Lu, Xuhua
• Yang, Li
• Chen, Ling-Ling

Titel

Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus

Ist Teil von

• Cell research, 2014-05, Vol.24 (5), p.513-531

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2014

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The human 8q24 gene desert contains multiple enhancers that form tissue-specific long-range chromatin loops with the MYC oncogene, but how chromatin looping at the MYC locus is regulated remains poorly understood. Here we demonstrate that a long noncoding RNA (lncRNA), CCAT1-L, is transcribed specifically in human colorectal cancers from a locus 515 kb upstream of MYC. This lncRNA plays a role in MYC transcriptional regulation and promotes long-range chromatin looping. Importantly, the CCAT1-L locus is located within a strong super-enhancer and is spatially close to MYC. Knockdown of CCAT1-L reduced long-range interactions between the MYC promoter and its enhancers. In addition, CCAT1-L interacts with CTCF and modulates chromatin conformation at these loop regions. These results reveal an important role of a previously unannotated lncRNA in gene regulation at the MYC locus.