Details

Autor(en) / Beteiligte

• Tong, Lin-jiang
• Xie, Hua
• Peng, Ting
• Liu, Xiao-feng
• Xin, Xian-liang
• Huang, Xun
• Chen, Si-meng
• Liu, Hong-yan
• Li, Hong-lin
• Geng, Mei-yu
• Yin, Ming
• Ding, Jian

Titel

Establishment of platform for screening insulin-like growth factor-1 receptor inhibitors and evaluation of novel inhibitors

Ist Teil von

• Acta pharmacologica Sinica, 2011-07, Vol.32 (7), p.930-938

Ort / Verlag

United States: Nature Publishing Group

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Aim： The insulin-like growth factor-1 receptor （IGFIR） is over-expressed in a wide variety of tumors and contributes to tumor cell proliferation, metastasis and drug resistance. The aim of this study was to establish a sensitive screening platform to identify novel IGFIR inhibitors. Methods： The catalytic domain of IGFIR was expressed using the Bac-to-Bac baculovirus expression system. The screening platform for IGFIR inhibitors was established based on ELISA. The binding profile of IGFIR with the inhibitors was predicted with molecular docking and then subjected to the surface plasmon resonance （SPR） approach. The growth inhibition of cancer cells by the inhibitors was assessed with MTT assay. Apoptosis was analyzed using flow cytometry and Western blotting. Results： A naturally occurring small molecule compound hematoxylin was identified as the most potent inhibitor （IC50 value=l.8±0.1 pmol/L） within a library of more than 200 compounds tested. Molecular simulation predicted the possible binding mode of hematoxylin with IGFIR. An SPR assay further confirmed that hematoxylin bound directly to IGFIR with high binding affinity （Kd=4.2×lO^-6 mol/L） In HL-60 cancer cells, hematoxylin inactivated the phosphorylation of IGFIR and downstream signaling and therefore suppressed cell proliferation. Mechanistic studies revealed that hematoxylin induced apoptosis in HL-60 cells via both extrinsic and intrinsic pathways. Conclusion： A simple, sensitive ELiSA-based screening platform for identifying IGFIR inhibitors was established. Hematoxylin was identified as a promising IGFIR inhibitor with effective antitumor activity that deserves further investigation.