Details

Autor(en) / Beteiligte

• Ba, Qian
• Duan, Juan
• Tian, Jia-qiang
• Wang, Zi-liang
• Chen, Tao
• Li, Xiao-guang
• Chen, Pei-zhan
• Wu, Song-jie
• Xiang, Li
• Li, Jing-quan
• Chu, Rui-ai
• Wang, Hui

Titel

Dihydroartemisinin promotes angiogenesis during the early embryonic development of zebrafish

Ist Teil von

• Acta pharmacologica Sinica, 2013-08, Vol.34 (8), p.1101-1107

Ort / Verlag

United States: Nature Publishing Group

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• Aim： To investigate the embryotoxicity of dihydroartemisinin （DHA）, the main active metabolite of artemisinin, in zebrafish, and explore the corresponding mechanisms. Methods： The embryos of wild type and TG （flkl：GFP） transgenic zebrafish were exposed to DHA. Developmental phenotypes of the embryos were observed. Development of blood vessels was directly observed in living embryos of TG （flkl：GFP） transgenic zebrafish under fluorescence microscope. The expression of angiogenesis marker genes vegfa, ilk1, and fit1 in the embryos was detected using real-time PCR and RNA in situ hybridization assays. Results： Exposure to DHA （1-10 mg/L） dose-dependently caused abnormal zebrafish embryonic phenotypes in the early developmental stage. Furthermore, exposure to DHA （10 mg/L） resulted in more pronounced embryonic angiogenesis in TG （flkl：GFP） zebrafish line. Exposure to DHA （10 mg/L） significantly increased the mRNA expression of veEfa, flkl, and fit1 in the embryos. Knockdown of the ilk1 protein partially blocked the effects of DHA on embryogenesis. Conclusion： DHA causes abnormal embryonic phenotypes and promotes angiogenesis in zebrafish early embryonic development, demonstrating the potential embryotoxicity of DHA.