Details

Autor(en) / Beteiligte

• Li, Cheng-tan
• Zhang, Wei-ping
• Fang, San-hua
• Lu, Yun-bi
• Zhang, Li-hui
• Qi, Ling-ling
• Huang, Xue-qin
• Huang, Xiao-jia
• Wei, Er-qing

Titel

Baicalin attenuates oxygen-glucose deprivation- induced injury by inhibiting oxidative stress-mediated 5-lipoxygenase activation in PC12 cells

Ist Teil von

• Acta pharmacologica Sinica, 2010-02, Vol.31 (2), p.137-144

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• Aim： To determine whether the flavonoid baicalin attenuates oxygen-glucose deprivation （OGD）-induced injury by inhibiting oxidative stress-mediated 5-1ipoxygenase （5-LOX） activation in PC12 cells. Methods： The effects of baicalin and the 5-LOX inhibitor zileuton on the changes induced by OGD/recovery or H2O2 （an exogenous reactive oxygen species [ROS]） in green fluorescent protein-5-LOX-transfected PC12 cells were compared. Results： Both baicalin and zileuton attenuated OGD/recovery- and H2O2-induced injury and inhibited OGD/recovery-induced production of 5-LOX metabolites （cysteinyl leukotrienes） in a concentration-dependent manner. However, baicalin did not reduce baseline cysteinyl leukotriene levels. Baicalin also reduced OGD/recovery-induced ROS production and inhibited 5-LOX translocation to the nuclear enve- lope and p38 phosphorylation induced by OGD/recovery and H2O2. In contrast, zileuton did not show these effects. Conclusion： Baicalin can inhibit 5-LOX activation after ischemic injury, which may partly result from inhibition of the ROS/p38 mitogenactivated protein kinase pathway.