Nature (London), 2014-04, Vol.508 (7497), p.550-553
2014
Details
- Autor(en) / Beteiligte
- Titel
- Structure of a type IV secretion system
- Ist Teil von
- Nature (London), 2014-04, Vol.508 (7497), p.550-553
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- EBSCOhost Psychology and Behavioral Sciences Collection
- Beschreibungen/Notizen
- Bacterial type IV secretion systems translocate virulence factors into eukaryotic cells, distribute genetic material between bacteria and have shown potential as a tool for the genetic modification of human cells. Given the complex choreography of the substrate through the secretion apparatus, the molecular mechanism of the type IV secretion system has proved difficult to dissect in the absence of structural data for the entire machinery. Here we use electron microscopy to reconstruct the type IV secretion system encoded by the Escherichia coli R388 conjugative plasmid. We show that eight proteins assemble in an intricate stoichiometric relationship to form an approximately 3 megadalton nanomachine that spans the entire cell envelope. The structure comprises an outer membrane-associated core complex connected by a central stalk to a substantial inner membrane complex that is dominated by a battery of 12 VirB4 ATPase subunits organized as side-by-side hexameric barrels. Our results show a secretion system with markedly different architecture, and consequently mechanism, to other known bacterial secretion systems.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0028-0836eISSN: 1476-4687DOI: 10.1038/nature13081Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3998870
- Format
- –
- Schlagworte
- Adenosine Triphosphatases, Adenosine Triphosphatases - chemistry, Adenosine Triphosphatases - genetics, Adenosine Triphosphatases - metabolism, Adenosine Triphosphatases - ultrastructure, Bacterial proteins, Bacterial Secretion Systems, Bacterial Secretion Systems - genetics, Bacteriology, Biochemistry, Molecular Biology, Bioinformatics, Biological Physics, Cell Membrane, Cell Membrane - metabolism, Cellular Biology, Chemical Sciences, Computer Science, Cristallography, E coli, Escherichia coli, Escherichia coli - chemistry, Escherichia coli - cytology, Escherichia coli - genetics, Escherichia coli - ultrastructure, Escherichia coli Proteins, Escherichia coli Proteins - chemistry, Escherichia coli Proteins - isolation & purification, Escherichia coli Proteins - metabolism, Escherichia coli Proteins - ultrastructure, Genetic research, Life Sciences, Microbiological research, Microscopy, Microscopy, Electron, Models, Molecular, Multiprotein Complexes, Multiprotein Complexes - chemistry, Multiprotein Complexes - genetics, Multiprotein Complexes - metabolism, Multiprotein Complexes - ultrastructure, Physics, Physiological aspects, Structural Biology, Symmetry, Translocation (Genetics), Virulence (Microbiology)