Details

Autor(en) / Beteiligte

• Ketley, Ami
• Chen, Catherine Z
• Li, Xin
• Arya, Sukrat
• Robinson, Thelma E
• Granados-Riveron, Javier
• Udosen, Inyang
• Morris, Glenn E
• Holt, Ian
• Furling, Denis
• Chaouch, Soraya
• Haworth, Ben
• Southall, Noel
• Shinn, Paul
• Zheng, Wei
• Austin, Christopher P
• Hayes, Christopher J
• Brook, J David

Titel

High-content screening identifies small molecules that remove nuclear foci, affect MBNL distribution and CELF1 protein levels via a PKC-independent pathway in myotonic dystrophy cell lines

Ist Teil von

• Human molecular genetics, 2014-03, Vol.23 (6), p.1551-1562

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

2014

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Myotonic dystrophy (DM) is a multi-system neuromuscular disorder for which there is no treatment. We have developed a medium throughput phenotypic assay, based on the identification of nuclear foci in DM patient cell lines using in situ hybridization and high-content imaging to screen for potentially useful therapeutic compounds. A series of further assays based on molecular features of DM have also been employed. Two compounds that reduce and/or remove nuclear foci have been identified, Ro 31-8220 and chromomycin A3. Ro 31-8220 is a PKC inhibitor, previously shown to affect the hyperphosphorylation of CELF1 and ameliorate the cardiac phenotype in a DM1 mouse model. We show that the same compound eliminates nuclear foci, reduces MBNL1 protein in the nucleus, affects ATP2A1 alternative splicing and reduces steady-state levels of CELF1 protein. We demonstrate that this effect is independent of PKC activity and conclude that this compound may be acting on alternative kinase targets within DM pathophysiology. Understanding the activity profile for this compound is key for the development of targeted therapeutics in the treatment of DM.