Details

Autor(en) / Beteiligte

• Chen, Baohui
• Gilbert, Luke A.
• Cimini, Beth A.
• Schnitzbauer, Joerg
• Zhang, Wei
• Li, Gene-Wei
• Park, Jason
• Blackburn, Elizabeth H.
• Weissman, Jonathan S.
• Qi, Lei S.
• Huang, Bo

Titel

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

Ist Teil von

• Cell, 2013-12, Vol.155 (7), p.1479-1491

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2013

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The spatiotemporal organization and dynamics of chromatin play critical roles in regulating genome function. However, visualizing specific, endogenous genomic loci remains challenging in living cells. Here, we demonstrate such an imaging technique by repurposing the bacterial CRISPR/Cas system. Using an EGFP-tagged endonuclease-deficient Cas9 protein and a structurally optimized small guide (sg) RNA, we show robust imaging of repetitive elements in telomeres and coding genes in living cells. Furthermore, an array of sgRNAs tiling along the target locus enables the visualization of nonrepetitive genomic sequences. Using this method, we have studied telomere dynamics during elongation or disruption, the subnuclear localization of the MUC4 loci, the cohesion of replicated MUC4 loci on sister chromatids, and their dynamic behaviors during mitosis. This CRISPR imaging tool has potential to significantly improve the capacity to study the conformation and dynamics of native chromosomes in living human cells. [Display omitted] •An optimized CRISPR enables live imaging and better gene regulation in human cells•CRISPR imaging visualizes either repetitive or nonrepetitive genomic sequences•CRISPR imaging reports telomere length change and telomere movements•CRISPR imaging monitors the dynamics of gene loci throughout the cell cycle A new CRISPR-based technology allows precise visualization of individual gene loci in living cells.