Details

Autor(en) / Beteiligte

• Dolatshahi-Pirouz, Alireza
• Nikkhah, Mehdi
• Gaharwar, Akhilesh K.
• Hashmi, Basma
• Guermani, Enrico
• Aliabadi, Hamed
• Camci-Unal, Gulden
• Ferrante, Thomas
• Foss, Morten
• Ingber, Donald E.
• Khademhosseini, Ali

Titel

A combinatorial cell-laden gel microarray for inducing osteogenic differentiation of human mesenchymal stem cells

Ist Teil von

• Scientific reports, 2014-01, Vol.4 (1), p.3896-3896

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2014

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Development of three dimensional (3D) microenvironments that direct stem cell differentiation into functional cell types remains a major challenge in the field of regenerative medicine. Here, we describe a new platform to address this challenge by utilizing a robotic microarray spotter for testing stem cell fates inside various miniaturized cell-laden gels in a systematic manner. To demonstrate the feasibility of our platform, we evaluated the osteogenic differentiation of human mesenchymal stem cells (hMSCs) within combinatorial 3D niches. We were able to identify specific combinations, that enhanced the expression of osteogenic markers. Notably, these ‘hit’ combinations directed hMSCs to form mineralized tissue when conditions were translated to 3D macroscale hydrogels, indicating that the miniaturization of the experimental system did not alter stem cell fate. Overall, our findings confirmed that the 3D cell-laden gel microarray can be used for screening of different conditions in a rapid, cost-effective and multiplexed manner for a broad range of tissue engineering applications.