Nature biotechnology, 2012-12, Vol.30 (12), p.1232-1239
- GANG FANG
- MUNERA, Diana
- DEIKUS, Gintaras
- CLARK, Tyson A
- LUONG, Khai
- MURRAY, Lain A
- DAVIS, Brigid M
- KEREN-PAZ, Alona
- CHESS, Andrew
- ROBERTS, Richard J
- KORLACH, Jonas
- TURNER, Steve W
- FRIEDMAN, David I
- KUMAR, Vipin
- WALDOR, Matthew K
- SCHADT, Eric E
- MANDLIK, Anjali
- CHAO, Michael C
- BANERJEE, Onureena
- ZHIXING FENG
- LOSIC, Bojan
- MAHAJAN, Milind C
- JABADO, Omar J
2012
Details
- Autor(en) / Beteiligte
- GANG FANG
- MUNERA, Diana
- DEIKUS, Gintaras
- CLARK, Tyson A
- LUONG, Khai
- MURRAY, Lain A
- DAVIS, Brigid M
- KEREN-PAZ, Alona
- CHESS, Andrew
- ROBERTS, Richard J
- KORLACH, Jonas
- TURNER, Steve W
- FRIEDMAN, David I
- KUMAR, Vipin
- WALDOR, Matthew K
- SCHADT, Eric E
- MANDLIK, Anjali
- CHAO, Michael C
- BANERJEE, Onureena
- ZHIXING FENG
- LOSIC, Bojan
- MAHAJAN, Milind C
- JABADO, Omar J
- Titel
- Genome-wide mapping of methylated adenine residues in pathogenic Escherichia coli using single-molecule real-time sequencing
- Ist Teil von
- Nature biotechnology, 2012-12, Vol.30 (12), p.1232-1239
- Ort / Verlag
- New York, NY: Nature Publishing Group
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Single-molecule real-time (SMRT) DNA sequencing allows the systematic detection of chemical modifications such as methylation but has not previously been applied on a genome-wide scale. We used this approach to detect 49,311 putative 6-methyladenine (m6A) residues and 1,407 putative 5-methylcytosine (m5C) residues in the genome of a pathogenic Escherichia coli strain. We obtained strand-specific information for methylation sites and a quantitative assessment of the frequency of methylation at each modified position. We deduced the sequence motifs recognized by the methyltransferase enzymes present in this strain without prior knowledge of their specificity. Furthermore, we found that deletion of a phage-encoded methyltransferase-endonuclease (restriction-modification; RM) system induced global transcriptional changes and led to gene amplification, suggesting that the role of RM systems extends beyond protecting host genomes from foreign DNA.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1087-0156eISSN: 1546-1696DOI: 10.1038/nbt.2432Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3879109
- Format
- –
- Schlagworte
- 5-Methylcytosine - metabolism, Adenine, Adenine - analogs & derivatives, Adenine - metabolism, Bacteriology, Biological and medical sciences, Biotechnology, Chromosome Mapping, DNA methylation, DNA Methylation - genetics, DNA Restriction-Modification Enzymes - deficiency, DNA Restriction-Modification Enzymes - genetics, DNA Restriction-Modification Enzymes - metabolism, DNA sequencing, DNA, Bacterial - chemistry, DNA, Bacterial - genetics, DNA, Bacterial - metabolism, E coli, Enzymes, Escherichia coli, Escherichia coli - genetics, Escherichia coli - metabolism, Escherichia coli - pathogenicity, Fundamental and applied biological sciences. Psychology, Gene Amplification, Gene Deletion, Genetic aspects, Genetics, Genome, Bacterial, Genome-wide association studies, Genomics, Microbiology, Nucleotide sequencing, Sequence Analysis, DNA - methods, Spiro Compounds, Substrate Specificity