Proceedings of the National Academy of Sciences - PNAS, 1986-08, Vol.83 (15), p.5607-5610
1986
Details
- Autor(en) / Beteiligte
- Titel
- Suppression of Human DNA Alkylation-Repair Defects by Escherichia coli DNA-Repair Genes
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1986-08, Vol.83 (15), p.5607-5610
- Ort / Verlag
- Washington, DC: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1986
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- The ada-alkB operon protects Escherichia coli against the effects of many alkylating agents. We have subcloned it into the pSV2 mammalian expression vector to yield pSV2ada-alkB, and this plasmid has been introduced into Mer- HeLa S3 cells, which are extremely sensitive to killing and induction of sister chromatid exchange by alkylating agents. One transformant (the S3-9 cell line) has several integrated copies of pSV2ada-alkB and was found to express a very high level of the ada gene product, the 39-kDa O6-methylguanine-DNA methyltransferase. S3-9 cells were found to have become resistant to killing and induction of sister chromatid exchange by two alkylating agents, N-methyl-N′-nitro-N-nitrosoguanidine and N,N′-bis(2-chloroethyl)-N-nitrosourea. This shows that bacterial DNA alkylation-repair genes are able to suppress the alkylation-repair defects in human Mer- cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.83.15.5607Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_386337
- Format
- –
- Schlagworte
- Alkylating Agents - toxicity, Alkylation, Biological and medical sciences, Biotechnology, Bone marrow cells, Cell lines, Cloning, Molecular, Cultured cells, DNA, DNA Repair, Drug Resistance, Escherichia coli, Escherichia coli - enzymology, Escherichia coli - genetics, Fundamental and applied biological sciences. Psychology, Gels, Genetic engineering, Genetic technics, Genetic Vectors, HeLa Cells, Humans, Lesions, Methods. Procedures. Technologies, Methyltransferases - genetics, Molecular cloning, O-Methylguanine-DNA Methyltransferase, Operons, Phenotype, Phenotypes, Plasmids, Species Specificity, Suppression, Genetic