Details

Autor(en) / Beteiligte

• Gao, Hanlin
• Boustany, Rose-Mary N.
• Espinola, Janice A.
• Cotman, Susan L.
• Srinidhi, Lakshmi
• Antonellis, Kristen Auger
• Gillis, Tammy
• Qin, Xuebin
• Liu, Shumei
• Donahue, Leah R.
• Bronson, Roderick T.
• Faust, Jerry R.
• Stout, Derek
• Haines, Jonathan L.
• Lerner, Terry J.
• MacDonald, Marcy E.

Titel

Mutations in a Novel CLN6-Encoded Transmembrane Protein Cause Variant Neuronal Ceroid Lipofuscinosis in Man and Mouse

Ist Teil von

• American journal of human genetics, 2002-02, Vol.70 (2), p.324-335

Ort / Verlag

Chicago, IL: Elsevier Inc

Erscheinungsjahr

2002

Quelle

MEDLINE

Beschreibungen/Notizen

• The CLN6 gene that causes variant late-infantile neuronal ceroid lipofuscinosis (vLINCL), a recessively inherited neurodegenerative disease that features blindness, seizures, and cognitive decline, maps to 15q21-23. We have used multiallele markers spanning this ∼4-Mb candidate interval to reveal a core haplotype, shared in Costa Rican families with vLINCL but not in a Venezuelan kindred, that highlighted a region likely to contain the CLN6 defect. Systematic comparison of genes from the minimal region uncovered a novel candidate, FLJ20561, that exhibited DNA sequence changes specific to the different disease chromosomes: a G→T transversion in exon 3, introducing a stop codon on the Costa Rican haplotype, and a codon deletion in exon 5, eliminating a conserved tyrosine residue on the Venezuelan chromosome. Furthermore, sequencing of the murine homologue in the nclf mouse, which manifests recessive NCL-like disease, disclosed a third lesion—an extra base pair in exon 4, producing a frameshift truncation on the nclf chromosome. Thus, the novel ∼36-kD CLN6-gene product augments an intriguing set of unrelated membrane-spanning proteins, whose deficiency causes NCL in mouse and man.