Genetics (Austin), 2013-11, Vol.195 (3), p.1157-1166
2013
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Analysis of allele-specific expression in mouse liver by RNA-Seq: a comparison with Cis-eQTL identified using genetic linkage
- Ist Teil von
- Genetics (Austin), 2013-11, Vol.195 (3), p.1157-1166
- Ort / Verlag
- United States: Genetics Society of America
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We report an analysis of allele-specific expression (ASE) and parent-of-origin expression in adult mouse liver using next generation sequencing (RNA-Seq) of reciprocal crosses of heterozygous F1 mice from the parental strains C57BL/6J and DBA/2J. We found a 60% overlap between genes exhibiting ASE and putative cis-acting expression quantitative trait loci (cis-eQTL) identified in an intercross between the same strains. We discuss the various biological and technical factors that contribute to the differences. We also identify genes exhibiting parental imprinting and complex expression patterns. Our study demonstrates the importance of biological replicates to limit the number of false positives with RNA-Seq data.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1943-2631, 0016-6731eISSN: 1943-2631DOI: 10.1534/genetics.113.153882Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3813844
- Format
- –
- Schlagworte
- Alleles, Animals, Antineoplastic Combined Chemotherapy Protocols, Crosses, Genetic, Cyclophosphamide, Etoposide, Female, Gene Expression, Genes, Genetic Linkage, Genomic Imprinting, Investigations, Life Sciences, Liver, Liver - metabolism, Male, Mice, Mice, Inbred C57BL, Mitoxantrone, Multigene Family, Polymorphism, Single Nucleotide, Prednisone, Quantitative Trait Loci, Ribonucleic acid, RNA, Rodents, Sequence Analysis, RNA