ACS synthetic biology, 2013-11, Vol.2 (11), p.629-634
2013
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Details
- Autor(en) / Beteiligte
- Titel
- Discovery of Cryptic Polyketide Metabolites from Dermatophytes Using Heterologous Expression in Aspergillus nidulans
- Ist Teil von
- ACS synthetic biology, 2013-11, Vol.2 (11), p.629-634
- Ort / Verlag
- United States: American Chemical Society
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Dermatophytes belonging to the Trichophyton and Arthroderma genera cause skin infections in humans and animals. From genome sequencing data, we mined a conserved gene cluster among dermatophytes that are homologous to one that produces an immunosuppressive polyketide in Aspergillus fumigatus. Using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. When integrated into the model Aspergillus nidulans host, a structurally related compound neosartoricin B was formed, suggesting a possible role of this compound in the pathogenesis of these strains.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2161-5063eISSN: 2161-5063DOI: 10.1021/sb400048bTitel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3795930
- Format
- –
- Schlagworte
- Arthrodermataceae - chemistry, Arthrodermataceae - genetics, Aspergillus nidulans - genetics, Aspergillus nidulans - metabolism, Cloning, Molecular, Fungal Proteins - genetics, Fungal Proteins - metabolism, Genes, Fungal, Multigene Family, Mutation, Polyketide Synthases - genetics, Polyketide Synthases - metabolism, Polyketides - metabolism, Secondary Metabolism