Details

Autor(en) / Beteiligte

• Lister, Ryan
• Mukamel, Eran A.
• Nery, Joseph R.
• Urich, Mark
• Puddifoot, Clare A.
• Johnson, Nicholas D.
• Lucero, Jacinta
• Huang, Yun
• Dwork, Andrew J.
• Schultz, Matthew D.
• Yu, Miao
• Tonti-Filippini, Julian
• Heyn, Holger
• Hu, Shijun
• Wu, Joseph C.
• Rao, Anjana
• Esteller, Manel
• He, Chuan
• Haghighi, Fatemeh G.
• Sejnowski, Terrence J.
• Behrens, M. Margarita
• Ecker, Joseph R.

Titel

Global Epigenomic Reconfiguration During Mammalian Brain Development

Ist Teil von

• Science (American Association for the Advancement of Science), 2013-08, Vol.341 (6146), p.629-629

Ort / Verlag

Washington, DC: American Association for the Advancement of Science

Erscheinungsjahr

2013

Quelle

American Association for the Advancement of Science

Beschreibungen/Notizen

• DNA methylation is implicated in mammalian brain development and plasticity underlying learning and memory. We report the genome-wide composition, patterning, cell specificity, and dynamics of DNA methylation at single-base resolution in human and mouse frontal cortex throughout their lifespan. Widespread methylome reconfiguration occurs during fetal to young adult development, coincident with synaptogenesis. During this period, highly conserved non-CG methylation (mCH) accumulates in neurons, but not glia, to become the dominant form of methylation in the human neuronal genome. Moreover, we found an mCH signature that identifies genes escaping X-chromosome inactivation. Last, whole-genome single-base resolution 5-hydroxymethylcytosine (hmC) maps revealed that hmC marks fetal brain cell genomes at putative regulatory regions that are CG-demethylated and activated in the adult brain and that CG demethylation at these hmC-poised loci depends on Tet2 activity.