Science (American Association for the Advancement of Science), 2003-11, Vol.302 (5649), p.1399-1401
2003
Details
- Autor(en) / Beteiligte
- Titel
- Nucleolar Clustering of Dispersed tRNA Genes
- Ist Teil von
- Science (American Association for the Advancement of Science), 2003-11, Vol.302 (5649), p.1399-1401
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2003
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Early transfer RNA (tRNA) processing events in Saccharomyces cerevisiae are coordinated in the nucleolus, the site normally associated with ribosome biosynthesis. To test whether spatial organization of the tRNA pathway begins with nucleolar clustering of the genes, we have probed the subnuclear location of five different tRNA gene families. The results show that tRNA genes, though dispersed in the linear genome, colocalize with 55 ribosomal DNA and U14 small nucleolar RNA at the nucleolus. Nucleolar localization requires tRNA gene transcription-complex formation, because inactivation of the promoter at a single locus removes its nucleolar association. This organization of tRNA genes must profoundly affect the spatial packaging of the genome and raises the question of whether gene types might be coordinated in three dimensions to regulate transcription.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1089814Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3783965
- Format
- –
- Schlagworte
- Biological and medical sciences, Cell nucleolus, Cell Nucleolus - genetics, Chromosomes, Coding, DNA Polymerase II - metabolism, DNA Polymerase III - metabolism, DNA, Ribosomal - analysis, DNA, Ribosomal - genetics, Eukaryotic cells, Fundamental and applied biological sciences. Psychology, Genes, Genes, Fungal, Genes, rRNA, Genetic transcription, Genome, Fungal, Genomes, Introns, Microorganisms, Molecular and cellular biology, Molecular genetics, Multigene Family, Prokaryotes, Promoter Regions, Genetic, Ribonucleic acid, RNA, RNA processing, RNA, Fungal - genetics, RNA, Ribosomal - biosynthesis, RNA, Ribosomal, 5S - genetics, RNA, Small Nucleolar - analysis, RNA, Transfer - genetics, RNA, Transfer, Gln - genetics, RNA, Transfer, Glu - genetics, RNA, Transfer, Gly - genetics, RNA, Transfer, Leu - genetics, RNA, Transfer, Lys - genetics, rRNA genes, Saccharomyces cerevisiae - genetics, Small nucleolar RNA, Transcription (Genetics), Transcription, Genetic, Transcription. Transcription factor. Splicing. Rna processing, Transfer RNA, Yeasts