Details

Autor(en) / Beteiligte

• MANSKE, Magnus
• MIOTTO, Olivo
• OUEDRAOGO, Jean-Bosco
• MICHON, Pascal
• MUELLER, Ivo
• SIBA, Peter
• NZILA, Alexis
• BORRMANN, Steffen
• KIARA, Steven M
• MARSH, Kevin
• HONGYING JIANG
• SU, Xin-Zhuan
• CAMPINO, Susana
• AMARATUNGA, Chanaki
• FAIRHURST, Rick
• SOCHEAT, Duong
• NOSTEN, Francois
• IMWONG, Mallika
• WHITE, Nicholas J
• SANDERS, Mandy
• ANASTASI, Elisa
• ALCOCK, Dan
• DRURYL, Eleanor
• AUBURN, Sarah
• OYOLA, Samuel
• QUAIL, Michael A
• TURNER, Daniel J
• RUANO-RUBIO, Valentin
• JYOTHI, Dushyanth
• AMENGA-ETEGO, Lucas
• HUBBART, Christina
• JEFFREYS, Anna
• ROWLANDS, Kate
• SUTHERLAND, Colin
• ALMAGRO-GARCIA, Jacob
• ROPER, Cally
• MANGANO, Valentina
• MODIANOL, David
• TAN, John C
• FERDIGL, Michael T
• AMAMBUA-NGWA, Alfred
• CONWAY, David J
• TAKALA-HARRISON, Shannon
• PLOWE, Christopher V
• RAYNER, Julian C
• MASLEN, Gareth
• ROCKETT, Kirk A
• CLARK, Taane G
• NEWBOLD, Chris I
• BERRIMAN, Matthew
• MACLNNIS, Bronwyn
• KWIATKOWSKI, Dominic P
• O'BRIEN, Jack
• DJIMDE, Abdoulaye
• DOUMBO, Ogobara
• ZONGO, Issaka

Titel

Analysis of Plasmodium falciparum diversity in natural infections by deep sequencing

Ist Teil von

• Nature (London), 2012-07, Vol.487 (7407), p.375-379

Ort / Verlag

London: Nature Publishing Group

Erscheinungsjahr

2012

Link zum Volltext

Quelle

EBSCOhost Psychology and Behavioral Sciences Collection

Beschreibungen/Notizen

• Malaria elimination strategies require surveillance of the parasite population for genetic changes that demand a public health response, such as new forms of drug resistance. Here we describe methods for the large-scale analysis of genetic variation in Plasmodium falciparum by deep sequencing of parasite DNA obtained from the blood of patients with malaria, either directly or after short-term culture. Analysis of 86,158 exonic single nucleotide polymorphisms that passed genotyping quality control in 227 samples from Africa, Asia and Oceania provides genome-wide estimates of allele frequency distribution, population structure and linkage disequilibrium. By comparing the genetic diversity of individual infections with that of the local parasite population, we derive a metric of within-host diversity that is related to the level of inbreeding in the population. An open-access web application has been established for the exploration of regional differences in allele frequency and of highly differentiated loci in the P. falciparum genome.