Molecular cell, 2013-04, Vol.50 (1), p.67-81
2013
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Details
- Autor(en) / Beteiligte
- Titel
- An Ancient Transcription Factor Initiates the Burst of piRNA Production during Early Meiosis in Mouse Testes
- Ist Teil von
- Molecular cell, 2013-04, Vol.50 (1), p.67-81
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2013
- Quelle
- Elektronische Zeitschriftenbibliothek
- Beschreibungen/Notizen
- Animal germ cells produce PIWI-interacting RNAs (piRNAs), small silencing RNAs that suppress transposons and enable gamete maturation. Mammalian transposon-silencing piRNAs accumulate early in spermatogenesis, whereas pachytene piRNAs are produced later during postnatal spermatogenesis and account for >95% of all piRNAs in the adult mouse testis. Mutants defective for pachytene piRNA pathway proteins fail to produce mature sperm, but neither the piRNA precursor transcripts nor the trigger for pachytene piRNA production is known. Here, we show that the transcription factor A-MYB initiates pachytene piRNA production. A-MYB drives transcription of both pachytene piRNA precursor RNAs and the mRNAs for core piRNA biogenesis factors including MIWI, the protein through which pachytene piRNAs function. A-MYB regulation of piRNA pathway proteins and piRNA genes creates a coherent feedforward loop that ensures the robust accumulation of pachytene piRNAs. This regulatory circuit, which can be detected in rooster testes, likely predates the divergence of birds and mammals. ► Mouse pachytene piRNA precursors are canonical RNA polymerase II transcripts ► A-MYB regulates transcription of pachytene piRNA-producing genes in spermatogenesis ► A-MYB activates genes encoding key proteins in the piRNA pathway, including MIWI ► A-MYB regulation creates a coherent feedforward loop, which is conserved in chicken
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1097-2765eISSN: 1097-4164DOI: 10.1016/j.molcel.2013.02.016Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3671569
- Format
- –
- Schlagworte
- adults, Animals, Argonaute Proteins - deficiency, Argonaute Proteins - genetics, biogenesis, Biological Evolution, Chickens, Endodeoxyribonucleases - deficiency, Endodeoxyribonucleases - genetics, Feedback, Physiological, Gene Expression Profiling, Gene Expression Regulation, Developmental, genes, Genotype, High-Throughput Nucleotide Sequencing, Male, Meiosis, messenger RNA, Mice, Mice, 129 Strain, Mice, Inbred C57BL, Mice, Knockout, mutants, Pachytene Stage, Phenotype, proteins, Proto-Oncogene Proteins c-myb - deficiency, Proto-Oncogene Proteins c-myb - genetics, Proto-Oncogene Proteins c-myb - metabolism, RNA, Small Interfering - biosynthesis, roosters, Spermatogenesis, spermatozoa, testes, Testis - growth & development, Testis - metabolism, Trans-Activators - deficiency, Trans-Activators - genetics, Trans-Activators - metabolism, transcription factors, Transcription, Genetic, Transcriptional Activation, transposons