Details

Autor(en) / Beteiligte

• Polando, Rachel
• Dixit, Upasna Gaur
• Carter, Cristina R.
• Jones, Blake
• Whitcomb, James P.
• Ballhorn, Wibke
• Harintho, Melissa
• Jerde, Christopher L.
• Wilson, Mary E.
• McDowell, Mary Ann

Titel

The roles of complement receptor 3 and Fcγ receptors during Leishmania phagosome maturation

Ist Teil von

• Journal of leukocyte biology, 2013-06, Vol.93 (6), p.921-932

Ort / Verlag

United States: Society for Leukocyte Biology

Erscheinungsjahr

2013

Quelle

Wiley Online Library All Journals

Beschreibungen/Notizen

• Opsonophagocytosis influences phagosomal trafficking of Leishmania without altering the intracellular fate. Leishmania are intracellular parasites adapted to surviving in macrophages, whose primary function is elimination of invading pathogens. Leishmania entry into host cells is receptor‐mediated. These parasites are able to engage multiple host cell‐surface receptors, including MR, TLRs, CR3, and FcγRs. Here, we investigated the role of CR3 and FcγR engagement on the maturation of Leishmania‐containing phagosomes using CD11b−/− and FcγR−/− macrophages, and assessing EEA1 and lysosome‐associated proteins is necessary for the phagosome maturation delay, characteristic of Leishmania infection. Leishmania‐containing phagosomes do not fuse with lyosomes until 5 h postinfection in WT mice. Phagolysosome fusion occurs by 1 h in CD11b and FcγR common chain KO macrophages, although receptor deficiency does not influence Leishmania entry or viability. We also investigated the influence of serum components and their effects on phagosome maturation progression. Opsonization with normal mouse serum, complement‐deficient serum, or serum from Leishmania‐infected mice all influenced phagosome maturation progression. Our results indicate that opsonophagocytosis influences phagosomal trafficking of Leishmania without altering the intracellular fate.