Details

Autor(en) / Beteiligte

• Wang, Li-ya
• Wang, Ning
• Le, Fang
• Li, Lei
• Li, Le-jun
• Liu, Xiao-zhen
• Zheng, Ying-ming
• Lou, Hang-ying
• Xu, Xiang-rong
• Zhu, Xiao-ming
• Zhu, Yi-min
• Huang, He-feng
• Jin, Fan

Titel

Persistence and intergenerational transmission of differentially expressed genes in the testes of intracytoplasmic sperm injection conceived mice

Ist Teil von

• Journal of Zhejiang University. B. Science, 2013-05, Vol.14 (5), p.372-381

Ort / Verlag

Heidelberg: SP Zhejiang University Press

Erscheinungsjahr

2013

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Intracytoplasmic sperm injection （ICSI） is commonly used to solve male infertility problems. Previous studies showed that early environmental exposure of an embryo may influence postnatal development. To detect whether ICSI operations affect the reproductive health of a male or his offspring, we established assisted reproductive technologies （ART） conceived mouse models, and analyzed gene expression profiles in the testes of both ICSI and naturally conceived （NC） newborn F~ mice using micro-array analysis. Among the differentially expressed genes, we focused on the expression of eight mate reproduction-related genes. Quantitative real-time reverse transcription- polymerase chain reaction （qRT-PCR） was used to analyze the expression of these genes in the testes of both adult and old F~ generation mice and adult F2 generation mice. Our results showed that down-regulated and somatic cell-expressed genes in newborn mice retained their differential expression patterns in adult and old F~ generation individuals, implying the persistence and fetal origin of the alteration in the expression of these genes. The intergen- erational transmission of differential gene expression was observed, but most changes tended to be reduced in adult F2 generations. Controlled ovarian hyperstimulation （COH） and in vitro fertilization （IVF） mice models were added to explore the precise factors contributing to the differences in ICSI offspring. The data demonstrated that superovulation, in vitro culture, and mechanical stimulation involved in ICSI had a cumulative effect on the differential expression of these male reproductive genes.