Scientific reports, 2013-02, Vol.3 (1), p.1188-1188, Article 1188
2013
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- Autor(en) / Beteiligte
- Titel
- Circulating human basophils lack the features of professional antigen presenting cells
- Ist Teil von
- Scientific reports, 2013-02, Vol.3 (1), p.1188-1188, Article 1188
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Recent reports in mice demonstrate that basophils function as antigen presenting cells (APC). They express MHC class II and co-stimulatory molecules CD80 and CD86, capture and present soluble antigens or IgE-antigen complexes and polarize Th2 responses. Therefore, we explored whether human circulating basophils possess the features of professional APC. We found that unlike dendritic cells (DC) and monocytes, steady-state circulating human basophils did not express HLA-DR and co-stimulatory molecules CD80 and CD86. Basophils remained negative for these molecules following stimulation with soluble Asp f 1, one of the allergens of Aspergillus fumigatus; Bet v 1, the major birch allergen; TLR2-ligand or even upon IgE cross-linking. Unlike DC, Asp f 1-pulsed basophils did not promote Th2 responses as analyzed by the secretion of IL-4 in the basophil-CD4(+) T cell co-culture. Together, these results demonstrate the inability of circulating human basophils to function as professional APC.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/srep01188Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3561623
- Format
- –
- Schlagworte
- Allergens, Allergens - genetics, Allergens - metabolism, Allergens - pharmacology, Antigen-presenting cells, Antigen-Presenting Cells - drug effects, Antigen-Presenting Cells - immunology, Antigen-Presenting Cells - metabolism, Antigens, Antigens, Plant - genetics, Antigens, Plant - metabolism, Antigens, Plant - pharmacology, B7-1 Antigen - metabolism, B7-2 Antigen - metabolism, Basophils - drug effects, Basophils - immunology, Basophils - metabolism, Bet v 1 antigen, CD4 antigen, CD4-Positive T-Lymphocytes - cytology, CD4-Positive T-Lymphocytes - immunology, CD80 antigen, CD86 antigen, Cell culture, Coculture Techniques, Dendritic cells, Dendritic Cells - drug effects, Dendritic Cells - immunology, Dendritic Cells - metabolism, Fungal Proteins - genetics, Fungal Proteins - metabolism, Fungal Proteins - pharmacology, Histocompatibility antigen HLA, HLA-DR Antigens - metabolism, Humans, Immunoglobulin E, Immunology, Innate immunity, Interleukin 4, Interleukin-4 - metabolism, Leukocytes (basophilic), Life Sciences, Lymphocytes T, Major histocompatibility complex, Monocytes, Monocytes - drug effects, Monocytes - immunology, Monocytes - metabolism, Recombinant Proteins - biosynthesis, Recombinant Proteins - genetics, Recombinant Proteins - pharmacology, TLR2 protein, Toll-like receptors