Details

Autor(en) / Beteiligte

• Tabansky, Inna
• Lenarcic, Alan
• Draft, Ryan W.
• Loulier, Karine
• Keskin, Derin B.
• Rosains, Jacqueline
• Rivera-Feliciano, José
• Lichtman, Jeff W.
• Livet, Jean
• Stern, Joel N.H.
• Sanes, Joshua R.
• Eggan, Kevin

Titel

Developmental Bias in Cleavage-Stage Mouse Blastomeres

Ist Teil von

• Current biology, 2013-01, Vol.23 (1), p.21-31

Ort / Verlag

England: Elsevier Inc

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• The cleavage-stage mouse embryo is composed of superficially equivalent blastomeres that will generate both the embryonic inner cell mass (ICM) and the supportive trophectoderm (TE). However, it remains unsettled whether the contribution of each blastomere to these two lineages can be accounted for by chance. Addressing the question of blastomere cell fate may be of practical importance, because preimplantation genetic diagnosis requires removal of blastomeres from the early human embryo. To determine whether blastomere allocation to the two earliest lineages is random, we developed and utilized a recombination-mediated, noninvasive combinatorial fluorescent labeling method for embryonic lineage tracing. When we induced recombination at cleavage stages, we observed a statistically significant bias in the contribution of the resulting labeled clones to the trophectoderm or the inner cell mass in a subset of embryos. Surprisingly, we did not find a correlation between localization of clones in the embryonic and abembryonic hemispheres of the late blastocyst and their allocation to the TE and ICM, suggesting that TE-ICM bias arises separately from embryonic-abembryonic bias. Rainbow lineage tracing also allowed us to demonstrate that the bias observed in the blastocyst persists into postimplantation stages and therefore has relevance for subsequent development. The Rainbow transgenic mice that we describe here have allowed us to detect lineage-dependent bias in early development. They should also enable assessment of the developmental equivalence of mammalian progenitor cells in a variety of tissues. ► Mouse lines for Rainbow lineage show tracing in many tissues and developmental stages ► A fraction of blastocysts exhibited biased clone contribution to the TE/ICM ► TE/ICM bias was uncorrelated with embryonic/abembryonic distribution of clones ► Postimplantation embryos had skewed contribution of clones to distinct lineages