Molecular therapy, 2013-01, Vol.21 (1), p.185-191
2013
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Details
- Autor(en) / Beteiligte
- Titel
- Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells
- Ist Teil von
- Molecular therapy, 2013-01, Vol.21 (1), p.185-191
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Despite the therapeutic potential of nucleic acid drugs, their clinical application has been limited in part by a lack of appropriate delivery systems. Exosomes or microvesicles are small endosomally derived vesicles that are secreted by a variety of cell types and tissues. Here, we show that exosomes can efficiently deliver microRNA (miRNA) to epidermal growth factor receptor (EGFR)-expressing breast cancer cells. Targeting was achieved by engineering the donor cells to express the transmembrane domain of platelet-derived growth factor receptor fused to the GE11 peptide. Intravenously injected exosomes delivered let-7a miRNA to EGFR-expressing xenograft breast cancer tissue in RAG2–/– mice. Our results suggest that exosomes can be used therapeutically to target EGFR-expressing cancerous tissues with nucleic acid drugs.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1525-0016eISSN: 1525-0024DOI: 10.1038/mt.2012.180Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3538304
- Format
- –
- Schlagworte
- Animals, Base Sequence, Blotting, Western, Breast cancer, Breast Neoplasms - metabolism, Breast Neoplasms - therapy, Cell Line, Tumor, Coculture Techniques, Exosomes - metabolism, Female, Humans, Mice, MicroRNAs - administration & dosage, Microscopy, Immunoelectron, Original, Real-Time Polymerase Chain Reaction, Receptor, Epidermal Growth Factor - genetics, Reverse Transcriptase Polymerase Chain Reaction, RNA, Small Interfering