Details

Autor(en) / Beteiligte

• Moir, Susan
• De Ravin, Suk See
• Santich, Brian H.
• Kim, Jin Young
• Posada, Jacqueline G.
• Ho, Jason
• Buckner, Clarisa M.
• Wang, Wei
• Kardava, Lela
• Garofalo, Mary
• Marciano, Beatriz E.
• Manischewitz, Jody
• King, Lisa R.
• Khurana, Surender
• Chun, Tae-Wook
• Golding, Hana
• Fauci, Anthony S.
• Malech, Harry L.

Titel

Humans with chronic granulomatous disease maintain humoral immunologic memory despite low frequencies of circulating memory B cells

Ist Teil von

• Blood, 2012-12, Vol.120 (24), p.4850-4858

Ort / Verlag

Washington, DC: Elsevier Inc

Erscheinungsjahr

2012

Link zum Volltext

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• CD27+ memory B cells are reduced in the blood of patients with chronic granulomatous disease (CGD) for reasons and consequences that remain unclear. Here we confirm not only decreased CD27+ but also IgG+ B cells in the blood of CGD patients compared with healthy donors (HDs). However, among IgG+ B cells, the ratio of CD27− to CD27+ was significantly higher in CGD patients compared with HDs. Similar to conventional memory B cells, CD27−IgG+ B cells of CGD patients expressed activation markers and had undergone somatic hypermutation, albeit at levels lower than their CD27+ counterparts. Functional analyses revealed slight reductions in frequencies of total IgG but not influenza-specific memory B-cell responses, as measured by Elispot in CGD patients compared with HDs. Serum IgG levels and influenza-specific antibodies were also normal in these CGD patients. Finally, we provide evidence that influenza-specific memory B cells can be present within the CD27−IgG+ B-cell compartment. Together, these findings show that, despite reduced circulating CD27+ memory B cells, CGD patients maintain an intact humoral immunologic memory, with potential contribution from CD27− B cells.