The Plant journal : for cell and molecular biology, 2012-03, Vol.69 (6), p.967-977
2012
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Details
- Autor(en) / Beteiligte
- Titel
- CDKB2 is involved in mitosis and DNA damage response in rice
- Ist Teil von
- The Plant journal : for cell and molecular biology, 2012-03, Vol.69 (6), p.967-977
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 2012
- Quelle
- Access via Wiley Online Library
- Beschreibungen/Notizen
- DNA damage checkpoints delay mitotic cell‐cycle progression in response to DNA stress, stalling the cell cycle to allow time for repair. CDKB is a plant‐specific cyclin‐dependent kinase (CDK) that is required for the G2/M transition of the cell cycle. In Arabidopsis, DNA damage leads the degradation of CDKB2, and the subsequent G2 arrest gives cells time to repair damaged DNA. G2 arrest also triggers transition from the mitotic cycle to endoreduplication, leading to the presence of polyploid cells in many tissues. In contrast, in rice (Oryza sativa), polyploid cells are found only in the endosperm. It was unclear whether endoreduplication contributes to alleviating DNA damage in rice (Oryza sativa). Here, we show that DNA damage neither down‐regulates Orysa;CDKB2;1 nor induces endoreduplication in rice. Furthermore, we found increased levels of Orysa;CDKB2;1 protein upon DNA damage. These results suggest that CDKB2 functions differently in Arabidopsis and rice in response to DNA damage. Arabidopsis may adopt endoreduplication as a survival strategy under genotoxic stress conditions, but rice may enhance DNA repair capacity upon genotoxic stress. In addition, polyploid cells due to endomitosis were present in CDKB2;1 knockdown rice, suggesting an important role for Orysa;CDKB2;1 during mitosis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0960-7412eISSN: 1365-313XDOI: 10.1111/j.1365-313x.2011.04847.xTitel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3440594
- Format
- –
- Schlagworte
- Agrobacterium - genetics, Agrobacterium - metabolism, Arabidopsis, Biological and medical sciences, Bleomycin - pharmacology, CDKB2, Cell cycle, Cell cycle, cell proliferation, Cell division, Cell physiology, Comet Assay, Culture Media - metabolism, Cyclin-dependent kinase, Cyclin-Dependent Kinases - genetics, Cyclin-Dependent Kinases - metabolism, DNA, DNA Damage, DNA repair, endomitosis, endopolyploidy, Endoreduplication, Endosperm, Endosperm - genetics, Endosperm - metabolism, Flowers & plants, Fundamental and applied biological sciences. Psychology, Gene Knockdown Techniques, Genotoxicity, Kinases, Mitosis, Molecular and cellular biology, Original, Oryza - drug effects, Oryza - genetics, Oryza - metabolism, Oryza - radiation effects, Oryza sativa, Plant biology, Plant Leaves - genetics, Plant Leaves - metabolism, Plant Leaves - ultrastructure, Plant physiology and development, Plant Proteins - genetics, Plant Proteins - metabolism, Plants, Genetically Modified - drug effects, Plants, Genetically Modified - genetics, Plants, Genetically Modified - metabolism, Plants, Genetically Modified - radiation effects, Plasmids - genetics, Plasmids - metabolism, ploidy, Polyploidy, Rad51 Recombinase - genetics, Rad51 Recombinase - metabolism, Rice, RNA Interference, Stress, X-Rays