Journal of clinical pathology, 2012-07, Vol.65 (7), p.660-662
2012
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Details
- Autor(en) / Beteiligte
- Titel
- Comparison of the RNA-based EndoPredict multigene test between core biopsies and corresponding surgical breast cancer sections
- Ist Teil von
- Journal of clinical pathology, 2012-07, Vol.65 (7), p.660-662
- Ort / Verlag
- England: BMJ Publishing Group Ltd and Association of Clinical Pathologists
- Erscheinungsjahr
- 2012
- Quelle
- BMJ Journals Archiv - DFG Nationallizenzen
- Beschreibungen/Notizen
- AimThis study compared the perfomance of the RNA-based EndoPredict multigene test on core biopsies and surgical breast cancer specimens and analysed the influence of biopsy-induced tissue injuries on the test result.Methods80 formalin-fixed paraffin-embedded samples comprising paired biopsies and surgical specimens from 40 ER-positive, HER2-negative patients were evaluated. Total RNA was extracted and the EndoPredict score was determined.ResultsRNA yield was considerably lower in core biopsies, but sufficient to measure the assay in all samples. The EndoPredict score was highly correlated between paired samples (Pearson r=0.92), with an excellent concordance of classification into a low or high risk of metastasis (overall agreement 95%).ConclusionsThe measurements are comparable between core biopsies and surgical sections, which suggest that the EndoPredict assay can be performed on core biopsy tissue. Inflammatory changes induced by presurgical biopsies had no significant effect on the RNA-based risk assessment in surgical specimens.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9746eISSN: 1472-4146DOI: 10.1136/jclinpath-2012-200716Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3426896
- Format
- –
- Schlagworte
- Biopsy - adverse effects, Breast, Breast - injuries, Breast - pathology, Breast cancer, Breast Neoplasms - genetics, Breast Neoplasms - metabolism, Breast Neoplasms - surgery, cancer, cancer genetics, cancer research, Carcinoma, Ductal - genetics, Carcinoma, Ductal - metabolism, Carcinoma, Ductal - surgery, Carcinoma, Lobular - genetics, Carcinoma, Lobular - metabolism, Carcinoma, Lobular - surgery, Diagnostic Tests, Routine - methods, EGFR, endocrine pathology, Endocrine therapy, Female, Gene expression, gynaecological pathology, Humans, Inflammation, Injuries, molecular oncology, molecular pathology, Multigene Family - genetics, oncology, ovary, Receptor, ErbB-2 - genetics, Receptor, ErbB-2 - metabolism, Receptors, Estrogen - genetics, Receptors, Estrogen - metabolism, Retrospective Studies, Risk Assessment, RNA, Neoplasm - genetics, RNA, Neoplasm - metabolism, Short Report, statistics, tumour markers, Wound healing