Genesis (New York, N.Y. : 2000), 2011-11, Vol.49 (11), p.841-850
2011
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- Autor(en) / Beteiligte
- Titel
- An in vivo map of bone morphogenetic protein 2 post-transcriptional repression in the heart
- Ist Teil von
- Genesis (New York, N.Y. : 2000), 2011-11, Vol.49 (11), p.841-850
- Ort / Verlag
- Hoboken: Wiley Subscription Services, Inc., A Wiley Company
- Erscheinungsjahr
- 2011
- Quelle
- Wiley Online Library - AutoHoldings Journals
- Beschreibungen/Notizen
- The Bmp2 3′untranslated region (UTR) sequence bears a sequence conserved between mammals and fishes that can post‐transcriptionally activate or repress protein synthesis. We developed a map of embryonic cells in the mouse where this potent Bmp2 regulatory sequence functions by using a lacZ reporter transgene with a 3′UTR bearing two loxP sites flanking the ultra‐conserved sequence. Cre‐recombinase‐mediated deletion of the ultra‐conserved sequence caused strong ectopic expression in proepicardium, epicardium and epicardium‐derived cells (EPDC) and in tissues with known epicardial contributions (coronary vessels and valves). Transient transfections of reporters in the epicardial/mesothelial cell (EMC) line confirmed this repression. Ectopic expression of the recombined transgene also occurred in the aorta, outlet septum, posterior cardiac plexus, cardiac and extracardiac nerves and neural ganglia. Bmp2 is dynamically regulated in the developing heart. 3′UTR‐mediated mechanisms that restrain BMP2 synthesis may be relevant to congenital heart and vasculature malformations and to adult diseases involving aberrant BMP2 synthesis. genesis 49:841–850, 2011. © 2011 Wiley‐Periodicals, Inc.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1526-954X, 1526-968XeISSN: 1526-968XDOI: 10.1002/dvg.20757Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3413373
- Format
- –
- Schlagworte
- 3' Untranslated Regions, Animals, Aorta, Bone morphogenetic protein 2, Bone Morphogenetic Protein 2 - genetics, Bone Morphogenetic Protein 2 - metabolism, Cell Line, Congenital defects, Conserved Sequence, coronary vasculature, Embryo, Mammalian - cytology, Embryo, Mammalian - metabolism, Embryo, Mammalian - physiology, Embryonic Development, Embryos, epicardial cells, Ganglia, Gene Expression Regulation, Developmental, gene regulation, Genes, Reporter, Heart, Heart - embryology, Heart - innervation, Heart - physiology, Immunohistochemistry, Integrases - metabolism, Lac Operon, Mice, Mice, Inbred C57BL, Mice, Transgenic - genetics, Mice, Transgenic - metabolism, mRNA, Nerves, neural crest, Neurofilament Proteins - genetics, Neurofilament Proteins - metabolism, Pericardium - cytology, Pericardium - embryology, Pericardium - metabolism, Pericardium - physiology, Post-transcription, post-transcriptional, Protein biosynthesis, Protein Processing, Post-Translational, Rats, Regulatory sequences, Regulatory Sequences, Nucleic Acid, Septum, Sequence Deletion, Transcription, Genetic, Transfection, Transgenes, transgenic mice