Clinical and experimental immunology, 2012-04, Vol.168 (1), p.68-74
2012
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- Autor(en) / Beteiligte
- Titel
- Excessive CD4+ T cells co‐expressing interleukin‐17 and interferon‐γ in patients with Behçet's disease
- Ist Teil von
- Clinical and experimental immunology, 2012-04, Vol.168 (1), p.68-74
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Summary Excessive T helper type 1 (Th1) cell activity has been reported in Behçet's disease (BD). Recently, association of Th17 cells with certain autoimmune diseases was reported, and we thus investigated circulating Th17 cells in BD. CD4+CD45RO– (naive) T cells were cultured with Th0‐, Th1‐, Th2‐ and Th17‐related cytokines and antibodies, and their mRNA was studied by real‐time polymerase chain reaction (PCR). When naive CD4+ T cells were cultured with Th1‐ and Th17‐related cytokines, interferon (IFN)‐γ mRNA and interleukin (IL)‐17 mRNA were up‐regulated, respectively, in BD patients. Naive CD4+ T cells cultured in a Th17 cell‐inducing condition expressed IL‐23 receptor (IL‐23R) mRNA excessively. IL‐17 mRNA expression was induced only when naive CD4+T cells were cultured in the presence of IL‐23. CD4+ T cells cultured with Th17 cytokines expressed excessive RAR‐related orphan receptor C (RORC) mRNA. Using intracellular cytokine staining, we found that CD45RO+(memory) CD4+ T cells producing IL‐17 and IFN‐γ simultaneously were increased significantly. Memory CD4+ T cells producing IFN‐γ but not IL‐17 decreased profoundly in BD patients. CD4+ T cells producing IL‐17 and IFN‐γ simultaneously were found in BD skin lesions. Collectively, we found excessive CD4+ T cells producing IL‐17 and IFN‐γ (Th1/Th17) cells in patients with BD, and possible involvement of IL‐23/IL‐23R pathway for the appearance of excessive Th1/Th17 cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0009-9104eISSN: 1365-2249DOI: 10.1111/j.1365-2249.2011.04543.xTitel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3390496
- Format
- –
- Schlagworte
- Adult, Analytical, structural and metabolic biochemistry, Antibodies, Autoimmune diseases, Behcet Syndrome - immunology, Behcet Syndrome - metabolism, Behcet Syndrome - pathology, Behcet's syndrome, Behçet's disease, Biological and medical sciences, CD4 antigen, CD4-Positive T-Lymphocytes - immunology, CD4-Positive T-Lymphocytes - metabolism, Cells, Cultured, cytokines/interleukins, Female, Fundamental and applied biological sciences. Psychology, gamma -Interferon, Gene expression, Helper cells, Humans, Immunological memory, Interferon-gamma - biosynthesis, Interleukin 17, Interleukin 23, Interleukin-17 - biosynthesis, Interleukin-17 - immunology, Interleukin-17 - metabolism, Interleukin-23 - biosynthesis, Interleukin-23 - immunology, Interleukin-23 - metabolism, Leukocyte Common Antigens - genetics, Lymphocytes T, Male, Medical sciences, Memory cells, Middle Aged, Nuclear Receptor Subfamily 1, Group F, Member 3 - biosynthesis, Original, Orphan receptors, Polymerase chain reaction, RNA, Messenger - biosynthesis, RNA, Messenger - genetics, Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis, Skin - immunology, Skin - pathology, Skin diseases, Th1 Cells - immunology, Th1 Cells - metabolism, Th17 cells, Th17 Cells - immunology