Details

Autor(en) / Beteiligte

• Ohzeki, Jun-ichirou
• Bergmann, Jan H
• Kouprina, Natalay
• Noskov, Vladimir N
• Nakano, Megumi
• Kimura, Hiroshi
• Earnshaw, William C
• Larionov, Vladimir
• Masumoto, Hiroshi

Titel

Breaking the HAC Barrier: Histone H3K9 acetyl/methyl balance regulates CENP-A assembly

Ist Teil von

• The EMBO journal, 2012-05, Vol.31 (10), p.2391-2402

Ort / Verlag

Chichester, UK: John Wiley & Sons, Ltd

Erscheinungsjahr

2012

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• The kinetochore is responsible for accurate chromosome segregation. However, the mechanism by which kinetochores assemble and are maintained remains unclear. Here we report that de novo CENP‐A assembly and kinetochore formation on human centromeric alphoid DNA arrays is regulated by a histone H3K9 acetyl/methyl balance. Tethering of histone acetyltransferases (HATs) to alphoid DNA arrays breaks a cell type‐specific barrier for de novo stable CENP‐A assembly and induces assembly of other kinetochore proteins at the ectopic alphoid site. Similar results are obtained following tethering of CENP‐A deposition factors hMis18α or HJURP. HAT tethering bypasses the need for hMis18α, but HJURP is still required for de novo kinetochore assembly. In contrast, H3K9 methylation following tethering of H3K9 tri‐methylase (Suv39h1) to the array prevents de novo CENP‐A assembly and kinetochore formation. CENP‐A arrays assembled de novo by this mechanism can form human artificial chromosomes (HACs) that are propagated indefinitely in human cells. Establishment of Human Artificial Chromosomes (HACs) depends on an interplay of H3 lysine 9 modifications at centromeres, providing insights into the pathways that control incorporation of the kinetochore‐specificing histone H3 variant CENP‐A.