The Journal of neuroscience, 2012-04, Vol.32 (16), p.5398-5413
2012
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Distinct subsets of Syt-IV/BDNF vesicles are sorted to axons versus dendrites and recruited to synapses by activity
- Ist Teil von
- The Journal of neuroscience, 2012-04, Vol.32 (16), p.5398-5413
- Ort / Verlag
- United States: Society for Neuroscience
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- BDNF plays a critical role in the regulation of synaptic strength and is essential for long-term potentiation, a phenomenon that underlies learning and memory. However, whether BDNF acts in a diffuse manner or is targeted to specific neuronal subcompartments or synaptic sites to affect circuit function remains unknown. Here, using photoactivation of BDNF or syt-IV (a regulator of exocytosis present on BDNF-containing vesicles) in transfected rat hippocampal neurons, we discovered that distinct subsets of BDNF vesicles are targeted to axons versus dendrites and are not shared between these compartments. Moreover, syt-IV- and BDNF-harboring vesicles are recruited to both presynaptic and postsynaptic sites in response to increased neuronal activity. Finally, using syt-IV knockout mouse neurons, we found that syt-IV is necessary for both presynaptic and postsynaptic scaling of synaptic strength in response to changes in network activity. These findings demonstrate that BDNF-containing vesicles can be targeted to specific sites in neurons and suggest that syt-IV-regulated BDNF secretion is subject to spatial control to regulate synaptic function in a site-specific manner.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0270-6474eISSN: 1529-2401DOI: 10.1523/jneurosci.4515-11.2012Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3352930
- Format
- –
- Schlagworte
- Activated-Leukocyte Cell Adhesion Molecule - metabolism, Animals, Animals, Newborn, Axons - metabolism, Brain-Derived Neurotrophic Factor - genetics, Brain-Derived Neurotrophic Factor - metabolism, Cells, Cultured, Coculture Techniques, Colforsin - pharmacology, Dendrites - metabolism, Disks Large Homolog 4 Protein, Embryo, Mammalian, Excitatory Amino Acid Agents - pharmacology, Excitatory Postsynaptic Potentials - drug effects, Excitatory Postsynaptic Potentials - genetics, Female, Glycine - pharmacology, Hippocampus - cytology, Humans, Intracellular Signaling Peptides and Proteins - metabolism, Luminescent Proteins - genetics, Luminescent Proteins - metabolism, Male, Membrane Proteins - metabolism, Mice, Mice, Knockout, Microtubule-Associated Proteins - metabolism, Neurons - cytology, Neurons - metabolism, Patch-Clamp Techniques, Rats, Receptors, AMPA - metabolism, Sodium Channel Blockers - pharmacology, Synapses - physiology, Synaptic Vesicles - classification, Synaptic Vesicles - metabolism, Synaptophysin - metabolism, Synaptotagmins - deficiency, Synaptotagmins - metabolism, Tetrodotoxin - pharmacology, Time Factors, Transfection, Vesicular Glutamate Transport Protein 1 - metabolism, Vesicular Inhibitory Amino Acid Transport Proteins - metabolism