Details

Autor(en) / Beteiligte

• Chaumeil, Myriam M
• Valette, Julien
• Baligand, Céline
• Brouillet, Emmanuel
• Hantraye, Philippe
• Bloch, Gilles
• Gaura, Véronique
• Rialland, Amandine
• Krystkowiak, Pierre
• Verny, Christophe
• Damier, Philippe
• Remy, Philippe
• Bachoud-Levi, Anne-Catherine
• Carlier, Pierre
• Lebon, Vincent

Titel

pH as a Biomarker of Neurodegeneration in Huntington's Disease: A Translational Rodent-Human MRS Study

Ist Teil von

• Journal of cerebral blood flow and metabolism, 2012-05, Vol.32 (5), p.771-779

Ort / Verlag

London, England: SAGE Publications

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• Early diagnosis and follow-up of neurodegenerative diseases are often hampered by the lack of reliable biomarkers. Neuroimaging techniques like magnetic resonance spectroscopy (MRS) offer promising tools to detect biochemical alterations at early stages of degeneration. Intracellular pH, which can be measured noninvasively by 31P-MRS, has shown variations in several brain diseases. Our purpose has been to evaluate the potential of MRS-measured pH as a relevant biomarker of early degeneration in Huntington's disease (HD). We used a translational approach starting with a preclinical validation of our hypothesis before adapting the method to HD patients. 31P-MRS-derived cerebral pH was first measured in rodents during chronic intoxication with 3-nitropropionic acid (3NP). A significant pH increase was observed early into the intoxication protocol (pH = 7.17 ± 0.02 after 3 days) as compared with preintoxication (pH = 7.08 ± 0.03). Furthermore, pH changes correlated with the 3NP-induced inhibition of succinate dehydrogenase and preceded striatum lesions. Using a similar MRS approach implemented on a clinical MRI, we then showed that cerebral pH was significantly higher in HD patients (n = 7) than in healthy controls (n = 6) (7.05 ± 0.03 versus 7.02 ± 0.01, respectively, P = 0.026). Altogether, both preclinical and human data strongly argue in favor of MRS-measured pH being a promising biomarker for diagnosis and follow-up of HD.