Details

Autor(en) / Beteiligte

• Walker, Laura M
• Phogat, Sanjay K
• Chan-Hui, Po-Ying
• Wagner, Denise
• Phung, Pham
• Goss, Julie L
• Wrin, Terri
• Simek, Melissa D
• Fling, Steven
• Mitcham, Jennifer L
• Lehrman, Jennifer K
• Priddy, Frances H
• Olsen, Ole A
• Frey, Steven M
• Hammond, Phillip W
• Kaminsky, Stephen
• Zamb, Timothy
• Moyle, Matthew
• Koff, Wayne C
• Poignard, Pascal
• Burton, Dennis R

Titel

Broad and Potent Neutralizing Antibodies from an African Donor Reveal a New HIV-1 Vaccine Target

Ist Teil von

• Science (American Association for the Advancement of Science), 2009-10, Vol.326 (5950), p.285-289

Ort / Verlag

Washington, DC: American Association for the Advancement of Science

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• Broadly neutralizing antibodies (bNAbs), which develop over time in some HIV-1-infected individuals, define critical epitopes for HIV vaccine design. Using a systematic approach, we have examined neutralization breadth in the sera of about 1800 HIV-1-infected individuals, primarily infected with non-clade B viruses, and have selected donors for monoclonal antibody (mAb) generation. We then used a high-throughput neutralization screen of antibody-containing culture supernatants from about 30,000 activated memory B cells from a clade A-infected African donor to isolate two potent mAbs that target a broadly neutralizing epitope. This epitope is preferentially expressed on trimeric Envelope protein and spans conserved regions of variable loops of the gp120 subunit. The results provide a framework for the design of new vaccine candidates for the elicitation of bNAb responses.