Details

Autor(en) / Beteiligte

• Takeuchi, Takeshi
• Kawashima, Takeshi
• Koyanagi, Ryo
• Gyoja, Fuki
• Tanaka, Makiko
• Ikuta, Tetsuro
• Shoguchi, Eiichi
• Fujiwara, Mayuki
• Shinzato, Chuya
• Hisata, Kanako
• Fujie, Manabu
• Usami, Takeshi
• Nagai, Kiyohito
• Maeyama, Kaoru
• Okamoto, Kikuhiko
• Aoki, Hideo
• Ishikawa, Takashi
• Masaoka, Tetsuji
• Fujiwara, Atushi
• Endo, Kazuyoshi
• Endo, Hirotoshi
• Nagasawa, Hiromichi
• Kinoshita, Shigeharu
• Asakawa, Shuichi
• Watabe, Shugo
• Satoh, Nori

Titel

Draft Genome of the Pearl Oyster Pinctada fucata: A Platform for Understanding Bivalve Biology

Ist Teil von

• DNA research, 2012-04, Vol.19 (2), p.117-130

Ort / Verlag

Oxford: Oxford University Press

Erscheinungsjahr

2012

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The study of the pearl oyster Pinctada fucata is key to increasing our understanding of the molecular mechanisms involved in pearl biosynthesis and biology of bivalve molluscs. We sequenced ∼1150-Mb genome at ∼40-fold coverage using the Roche 454 GS-FLX and Illumina GAIIx sequencers. The sequences were assembled into contigs with N50 = 1.6 kb (total contig assembly reached to 1024 Mb) and scaffolds with N50 = 14.5 kb. The pearl oyster genome is AT-rich, with a GC content of 34%. DNA transposons, retrotransposons, and tandem repeat elements occupied 0.4, 1.5, and 7.9% of the genome, respectively (a total of 9.8%). Version 1.0 of the P. fucata draft genome contains 23 257 complete gene models, 70% of which are supported by the corresponding expressed sequence tags. The genes include those reported to have an association with bio-mineralization. Genes encoding transcription factors and signal transduction molecules are present in numbers comparable with genomes of other metazoans. Genome-wide molecular phylogeny suggests that the lophotrochozoan represents a distinct clade from ecdysozoans. Our draft genome of the pearl oyster thus provides a platform for the identification of selection markers and genes for calcification, knowledge of which will be important in the pearl industry.