Proceedings of the National Academy of Sciences - PNAS, 2012-03, Vol.109 (13), p.4863-4868
2012
Details
- Autor(en) / Beteiligte
- Titel
- Cellular solid-state nuclear magnetic resonance spectroscopy
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2012-03, Vol.109 (13), p.4863-4868
- Ort / Verlag
- United States: National Academy of Sciences
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Decrypting the structure, function, and molecular interactions of complex molecular machines in their cellular context and at atomic resolution is of prime importance for understanding fundamental physiological processes. Nuclear magnetic resonance is a well-established imaging method that can visualize cellular entities at the micrometer scale and can be used to obtain 3D atomic structures under in vitro conditions. Here, we introduce a solid-state NMR approach that provides atomic level insights into cell-associated molecular components. By combining dedicated protein production and labeling schemes with tailored solid-state NMR pulse methods, we obtained structural information of a recombinant integral membrane protein and the major endogenous molecular components in a bacterial environment. Our approach permits studying entire cellular compartments as well as cell-associated proteins at the same time and at atomic resolution.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.1116478109Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3323964
- Format
- –
- Schlagworte
- Amino Acid Sequence, Bacterial Outer Membrane Proteins - chemistry, Bacterial Outer Membrane Proteins - metabolism, Biological Sciences, Cell Membrane - metabolism, Cells, Correlations, digital images, Escherichia coli, Escherichia coli - cytology, Escherichia coli - metabolism, Escherichia coli Proteins - chemistry, Escherichia coli Proteins - metabolism, image analysis, Lipids, Lipopolysaccharides - chemistry, Lipopolysaccharides - metabolism, Lipoproteins, Lipoproteins - chemistry, Lipoproteins - metabolism, Magnetic Resonance Spectroscopy - methods, Membrane proteins, Molecular Sequence Data, Molecular structure, NMR, Nuclear magnetic resonance, nuclear magnetic resonance spectroscopy, Peptidoglycan - chemistry, Peptidoglycan - metabolism, Physiology, Protein Conformation, Proteins, Proteolipids - chemistry, Proteolipids - metabolism, Spectral correlation, Spectroscopic analysis, Spectroscopy