Science (American Association for the Advancement of Science), 2011-09, Vol.333 (6049), p.1637-1640
2011
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- Autor(en) / Beteiligte
- Titel
- Yeast Rrn7 and Human TAF1B Are TFIIB-Related RNA Polymerase I General Transcription Factors
- Ist Teil von
- Science (American Association for the Advancement of Science), 2011-09, Vol.333 (6049), p.1637-1640
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2011
- Quelle
- Science Online_科学在线
- Beschreibungen/Notizen
- Eukaryotic and archaeal multisubunit RNA polymerases (Pols) are structurally related and require several similar components for transcription initiation. However, none of the Pol I factors were known to share homology with transcription factor IIB (TFIIB) or TFIIB-related proteins, key factors in the initiation mechanisms of the other Pols. Here we show that Rrn7, a subunit of the yeast Pol I core factor, and its human ortholog TAF1B are TFIIB-like factors. Although distantly related, Rrn7 shares many activities associated with TFIIB-like factors. Domain swaps between TFIIB-related factors show that Rrn7 is most closely related to the Pol III general factor Brf1. Our results point to the conservation of initiation mechanisms among multisubunit Pols and reveal a key function of yeast core factor/human SL1 in Pol I transcription.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1207699Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3319074
- Format
- –
- Schlagworte
- Active sites, Amino Acid Sequence, Amino Acid Substitution, Biological and medical sciences, Biosynthesis, Brf1 protein, Cyclins, DNA, Docks, Enzymes, Eukaryotes, Fundamental and applied biological sciences. Psychology, General transcription factors, Genetic mutation, Humans, Molecular and cellular biology, Molecular genetics, Molecular Sequence Data, Pol1 Transcription Initiation Complex Proteins - chemistry, Pol1 Transcription Initiation Complex Proteins - genetics, Pol1 Transcription Initiation Complex Proteins - metabolism, Protein Folding, Protein Interaction Domains and Motifs, Protein Structure, Secondary, Protein Structure, Tertiary, Proteins, RNA, RNA polymerase, RNA Polymerase I - metabolism, Saccharomyces cerevisiae Proteins - chemistry, Saccharomyces cerevisiae Proteins - genetics, Saccharomyces cerevisiae Proteins - metabolism, Sequence Alignment, TATA-Box Binding Protein - metabolism, Transcription Factor TFIIB - chemistry, Transcription Factor TFIIB - metabolism, Transcription Factor TFIIIB - chemistry, Transcription Factor TFIIIB - genetics, Transcription Factor TFIIIB - metabolism, Transcription, Genetic, Transcription. Transcription factor. Splicing. Rna processing, Yeast, Yeasts