Details

Autor(en) / Beteiligte

• Wann, Angus K. T.
• Zuo, Ning
• Haycraft, Courtney J.
• Jensen, Cynthia G.
• Poole, C. Anthony
• McGlashan, Susan R.
• Knight, Martin M.

Titel

Primary cilia mediate mechanotransduction through control of ATP‐induced Ca2+ signaling in compressed chondrocytes

Ist Teil von

• The FASEB journal, 2012-04, Vol.26 (4), p.1663-1671

Ort / Verlag

Bethesda, MD, USA: Federation of American Societies for Experimental Biology

Erscheinungsjahr

2012

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• We investigated the role of the chondrocyte primary cilium in mechanotransduction events related to cartilage extracellular matrix synthesis. We generated conditionally immortalized wild‐type (WT) and IFT88orpk (ORPK) mutant chondrocytes that lack primary cilia and assessed intracellular Ca2+ signaling, extracellular matrix synthesis, and ATP release in response to physiologically relevant compressive strains in a 3‐dimensional chondrocyte culture system. All conditions were compared to unloaded controls. We found that cilia were required for compression‐induced Ca2+ signaling mediated by ATP release, and an associated up‐regulation of aggrecan mRNA and sulfated glycosaminosglycan secretion. However, chondrocyte cilia were not the initial mechanoreceptors, since both WT and ORPK cells showed mechanically induced ATP release. Rather, we found that primary cilia were required for downstream ATP reception, since ORPK cells did not elicit a Ca2+ response to exogenous ATP even though WT and ORPK cells express similar levels of purine receptors. We suggest that purinergic Ca2+ signaling may be regulated by polycystin‐1, since ORPK cells only expressed the C‐terminal tail. This is the first study to demonstrate that primary cilia are essential organelles for cartilage mechanotransduction, as well as identifying a novel role for primary cilia not previously reported in any other cell type, namely cilia‐mediated control of ATP reception.—Wann, A. K. T., Zuo, N., Haycraft, C. J., Jensen, C. G., Poole, C. A., McGlashan, S. R., Knight, M. M. Primary cilia mediate mechanotransduction through control of ATP‐induced Ca2+ signaling in compressed chondrocytes. FASEB J. 26, 1663‐1671 (2012). www.fasebj.org