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p68 is a conserved nuclear protein, first identified by its specific immunological crossreaction with the SV40 large tumor antigen. Recently, both proteins have been shown to possess RNA helicase activity. A full length cDNA (HPz) encoding the human p68 protein was isolated from a lambda gt11 library. The nucleotide sequence has been determined using the dideoxynucleotide chain termination method and the amino acid (aa) sequence deduced. The cDNA consists of 2309 nucleotides and shows an open reading frame encoding a polypeptide of 614 aa with a Mr. of 69034.