Details

Autor(en) / Beteiligte

• Ishikawa, Hiroaki
• Thompson, James
• Yates, John R.
• Marshall, Wallace F.

Titel

Proteomic Analysis of Mammalian Primary Cilia

Ist Teil von

• Current biology, 2012-03, Vol.22 (5), p.414-419

Ort / Verlag

England: Elsevier Inc

Erscheinungsjahr

2012

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• The primary cilium is a microtubule-based organelle that senses extracellular signals as a cellular antenna [1]. Primary cilia are found on many types of cells in our body and play important roles in development and physiology. Defects of primary cilia cause a broad class of human genetic diseases called ciliopathies. To gain new insights into ciliary functions and better understand the molecular mechanisms underlying ciliopathies, it is of high importance to generate a catalog of primary cilia proteins. In this study, we isolated primary cilia from mouse kidney cells by using a calcium-shock method and identified 195 candidate primary cilia proteins by MudPIT (multidimensional protein identification technology), protein correlation profiling, and subtractive proteomic analysis. Based on comparisons with other proteomic studies of cilia, around 75% of our candidate primary cilia proteins are shared components with motile or specialized sensory cilia. The remaining 25% of the candidate proteins are possible primary cilia-specific proteins. These possible primary cilia-specific proteins include EVC2, INPP5E, and inversin, several of which have been linked to known ciliopathies. We have performed the first reported proteomic analysis of primary cilia from mammalian cells. These results provide new insights into primary cilia structure and function. ► This is the first reported proteomic analysis of generic mammalian primary cilia ► 195 proteins were identified as components of generic mammalian primary cilia ► 75% of generic primary cilia proteins are also found in motile or sensory cilia ► Most IFT proteins are conserved in generic primary, sensory, and motile cilia